A SIMPLE METHOD FOR AFFINITY PURIFICATION OF RADIOLABELED MONOCLONAL-ANTIBODIES

被引：1
作者：
JUWEID, M
SATO, J
PAIK, C
ONAYBASARAN, S
WEINSTEIN, JN
NEUMANN, RD
机构：
[1] NCI, CTR CLIN,DEPT NUCL MED,10-1 C-496, 9000 ROCKVILLE PIKE, BETHESDA, MD 20892 USA
[2] NCI, MATH BIOL LAB, BETHESDA, MD 20892 USA
来源：
NUCLEAR MEDICINE AND BIOLOGY | 1993年 / 20卷 / 03期
关键词：
D O I：
10.1016/0969-8051(93)90053-W
中图分类号：
R8 [特种医学]; R445 [影像诊断学];
学科分类号：
1002 ; 100207 ; 1009 ;
摘要：
A simple method is described for affinity purification of radiolabeled antibodies using glutaraldehyde-fixed tumor target cells. The cell-bound antibody fraction is removed from the cells by an acid wash and then immediately subjected to buffer-exchange chromatography. The method was applied to the D3 murine monoclonal antibody which binds to a 290 kDa antigen on the surface of Line 10 guinea pig carcinoma cells. No alteration in the molecular size profile was detected after acid washing. Purification resulted in a significant increase in immunoreactivity by an average of 14 +/- 47% (SD; range 4-30%).
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页码：311 / 315
页数：5
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