SYNTHETIC DNA BENDING SEQUENCES INCREASE THE RATE OF INVITRO TRANSCRIPTION INITIATION AT THE ESCHERICHIA-COLI LAC-PROMOTER

被引：136
作者：
GARTENBERG, MR
CROTHERS, DM
机构：
[1] Department of Chemistry, Yule University New Haven
来源：
JOURNAL OF MOLECULAR BIOLOGY | 1991年 / 219卷 / 02期
关键词：
DNA BENDING; A-TRACTS; TRANSCRIPTION; LAC-PROMOTER; SUPERCOILED DNA;
D O I：
10.1016/0022-2836(91)90563-L
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Appropriately phased DNA bending sequences replacing the CAP binding site upstream from the lac promoter increase by roughly tenfold the rate of specific transcription initiation from a superhelical promoter template in vitro; promoter occlusion results from polymerase binding to the upstream (dA)n · (dT)n tracts, but this phenomenon is not responsible for the observed phase-dependent transcriptional activity. The rates of open complex formation at both P1 and P2 promoters respond in a similar phase-dependent way to the synthetic curved DNA sequences. © 1991.
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页码：217 / 230
页数：14
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共 51 条
[1]   BINDING OF CRP TO DNA-DEPENDENT RNA-POLYMERASE FROM ESCHERICHIA-COLI - MODULATION BY CAMP OF THE INTERACTIONS WITH FREE AND DNA-BOUND HOLO AND CORE ENZYME [J].
BLAZY, B ;
TAKAHASHI, M ;
BAUDRAS, A .
MOLECULAR BIOLOGY REPORTS, 1980, 6 (01) :39-43
[2]   SUPERCOILING RESPONSE OF THE LAC PS PROMOTER INVITRO [J].
BOROWIEC, JA ;
GRALLA, JD .
JOURNAL OF MOLECULAR BIOLOGY, 1985, 184 (04) :587-598
[3]   DNA SUPERCOILING PROMOTES FORMATION OF A BENT REPRESSION LOOP IN LAC DNA [J].
BOROWIEC, JA ;
LI, Z ;
SASSEDWIGHT, S ;
GRALLA, JD .
JOURNAL OF MOLECULAR BIOLOGY, 1987, 196 (01) :101-111
[4]   CONFORMATIONAL CHANGE IN THE DNA ASSOCIATED WITH AN UNUSUAL PROMOTER MUTATION IN A TRANSFER-RNA OPERON OF SALMONELLA [J].
BOSSI, L ;
SMITH, DM .
CELL, 1984, 39 (03) :643-652
[5]   SYNTHETIC CURVED DNA-SEQUENCES CAN ACT AS TRANSCRIPTIONAL ACTIVATORS IN ESCHERICHIA-COLI [J].
BRACCO, L ;
KOTLARZ, D ;
KOLB, A ;
DIEKMANN, S ;
BUC, H .
EMBO JOURNAL, 1989, 8 (13) :4289-4296
[6]   PROMOTER RECOGNITION AND PROMOTER STRENGTH IN THE ESCHERICHIA-COLI SYSTEM [J].
BRUNNER, M ;
BUJARD, H .
EMBO JOURNAL, 1987, 6 (10) :3139-3144
[7]   MECHANISM OF ACTIVATION OF TRANSCRIPTION BY THE COMPLEX FORMED BETWEEN CYCLIC-AMP AND ITS RECEPTOR IN ESCHERICHIA-COLI [J].
BUC, H .
BIOCHEMICAL SOCIETY TRANSACTIONS, 1986, 14 (02) :196-199
[8]   RNA-POLYMERASE MAKES IMPORTANT CONTACTS UPSTREAM FROM BASE PAIR 49 AT THE ESCHERICHIA-COLI GALACTOSE OPERON P1 PROMOTER [J].
BUSBY, S ;
SPASSKY, A ;
CHAN, B .
GENE, 1987, 53 (2-3) :145-152
[9]   FUNCTIONAL-EFFECTS OF CIS-THYMINE GLYCOL LESIONS ON DNA-SYNTHESIS INVITRO [J].
CLARK, JM ;
BEARDSLEY, GP .
BIOCHEMISTRY, 1987, 26 (17) :5398-5403
[10]   INFLUENCE OF THE SEQUENCE-DEPENDENT FLEXURE OF DNA ON TRANSCRIPTION IN ESCHERICHIA-COLI [J].
COLLIS, CM ;
MOLLOY, PL ;
BOTH, GW ;
DREW, HR .
NUCLEIC ACIDS RESEARCH, 1989, 17 (22) :9447-9468
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