MONOCLONAL-ANTIBODY TO PROTEOGLYCAN DERIVED FROM GRIFOLA-FRONDOSA (MAITAKE)

被引：0

作者：

HIRATA, A

ADACHI, Y

ITOH, W

KOMODA, M

TABATA, K

SUGAWARA, I

机构：

[1] TOKYO COLL PHARM, IMMUNOPHARMACOL MICROBIAL PROD LAB, HACHIOJI, TOKYO 19203, JAPAN

[2] SAITAMA MED SCH, SAITAMA MED CTR, DEPT PATHOL, KAWAGOE, SAITAMA 350, JAPAN

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 1994年 / 17卷 / 04期

关键词：

MONOCLONAL ANTIBODY; PROTEOGLYCAN; GRIFOLA FRONDOSA; SCHIZOPHYLLAN; ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA);

D O I：

暂无

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

A murine monoclonal antibody (MAb) was prepared by immunizing BALB/c mice with a proteoglycan fraction derived from Grifola frondosa (Maitake mushroom), followed by the hybridization of spleen cells with mouse myeloma cells. The MAb (subclass; Ig G(2b)), designated MPG2, reacted with schizophyllan (SPG), curdlan, scleroglucan, laminarin and lentinan, but not with dextran, pullulan, mannan and xylan. Immunohistochemistry (ABC-GO method) shelved that MAb MPG2 reacted with lysosomal proteoglycan and (1->6)-beta-branched laminaritriose taken up by rabbit peritoneal macrophages. These results suggest that this MAb may recognize mainly (1->3)-beta-D-glucan, and may be useful for determining the immunological properties of Grifola frondosa-derived proteoglycan.

引用

页码：539 / 542

页数：4

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