MONOCLONAL-ANTIBODY TO PROTEOGLYCAN DERIVED FROM GRIFOLA-FRONDOSA (MAITAKE)

被引：0
作者：
HIRATA, A
ADACHI, Y
ITOH, W
KOMODA, M
TABATA, K
SUGAWARA, I
机构：
[1] TOKYO COLL PHARM, IMMUNOPHARMACOL MICROBIAL PROD LAB, HACHIOJI, TOKYO 19203, JAPAN
[2] SAITAMA MED SCH, SAITAMA MED CTR, DEPT PATHOL, KAWAGOE, SAITAMA 350, JAPAN
来源：
BIOLOGICAL & PHARMACEUTICAL BULLETIN | 1994年 / 17卷 / 04期
关键词：
MONOCLONAL ANTIBODY; PROTEOGLYCAN; GRIFOLA FRONDOSA; SCHIZOPHYLLAN; ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA);
D O I：
暂无
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
A murine monoclonal antibody (MAb) was prepared by immunizing BALB/c mice with a proteoglycan fraction derived from Grifola frondosa (Maitake mushroom), followed by the hybridization of spleen cells with mouse myeloma cells. The MAb (subclass; Ig G(2b)), designated MPG2, reacted with schizophyllan (SPG), curdlan, scleroglucan, laminarin and lentinan, but not with dextran, pullulan, mannan and xylan. Immunohistochemistry (ABC-GO method) shelved that MAb MPG2 reacted with lysosomal proteoglycan and (1->6)-beta-branched laminaritriose taken up by rabbit peritoneal macrophages. These results suggest that this MAb may recognize mainly (1->3)-beta-D-glucan, and may be useful for determining the immunological properties of Grifola frondosa-derived proteoglycan.
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页码：539 / 542
页数：4
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