THE 8.5-ANGSTROM PROJECTION MAP OF THE LIGHT-HARVESTING COMPLEX-I FROM RHODOSPIRILLUM-RUBRUM REVEALS A RING COMPOSED OF 16 SUBUNITS

被引：555
作者：
KARRASCH, S
BULLOUGH, PA
GHOSH, R
机构：
[1] MRC,MOLEC BIOL LAB,CAMBRIDGE CB2 2QH,ENGLAND
[2] UNIV BASEL,BIOZENTRUM,DEPT MICROBIOL,CH-4056 BASEL,SWITZERLAND
来源：
EMBO JOURNAL | 1995年 / 14卷 / 04期
关键词：
ELECTRON CRYOMICROSCOPY; LHC I; PHOTOSYNTHESIS; RHODOSPIRILLUM RUBRUM; 2-D CRYSTALS;
D O I：
10.1002/j.1460-2075.1995.tb07041.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Two-dimensional crystals from light-harvesting complex I (LHC I) of the purple non-sulfur bacterium Rhodospirillum rubrum have been reconstituted from detergent-solubilized protein complexes. Frozen-hydrated samples have been analysed by electron microscopy. The crystals diffract beyond 8 Angstrom and a projection map was calculated to 8.5 Angstrom. The projection map shows 16 subunits in a 116 Angstrom diameter ring with a 68 Angstrom hole in the centre, These dimensions are sufficient to incorporate a reaction centre in vivo. Within each subunit, density for the alpha- and the beta-polypeptide chains is clearly resolved, and the density for the bacteriochlorophylls can be assigned. The experimentally determined structure contradicts models of the LHC I presented so far.
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收藏
页码：631 / 638
页数：8
相关论文
共 39 条
[11]   STOICHIOMETRIC MODEL OF THE PHOTOSYNTHETIC UNIT OF ECTOTHIORHODOSPIRA-HALOCHLORIS [J].
ENGELHARDT, H ;
ENGEL, A ;
BAUMEISTER, W .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (23) :8972-8976
[12]  
GALL A, 1994, THESIS U GLASGOW UK
[13]   2-DIMENSIONAL CRYSTALLIZATION OF THE LIGHT-HARVESTING COMPLEX FROM RHODOSPIRILLUM-RUBRUM [J].
GHOSH, R ;
HOENGER, A ;
HARDMEYER, A ;
MIHAILESCU, D ;
BACHOFEN, R ;
ENGEL, A ;
ROSENBUSCH, JP .
JOURNAL OF MOLECULAR BIOLOGY, 1993, 231 (02) :501-504
[14]   REVERSIBLE DISSOCIATION OF THE B873 LIGHT-HARVESTING COMPLEX FROM RHODOSPIRILLUM-RUBRUM G9+ [J].
GHOSH, R ;
HAUSER, H ;
BACHOFEN, R .
BIOCHEMISTRY, 1988, 27 (03) :1004-1014
[15]  
GHOSH R, 1990, MOL BIOL MEMBRANE BO, P245
[16]   CRYSTALLIZATION OF THE B800-820 LIGHT-HARVESTING COMPLEX FROM RHODOPSEUDOMONAS-ACIDOPHILA STRAIN 7750 [J].
GUTHRIE, N ;
MACDERMOTT, G ;
COGDELL, RJ ;
FREER, AA ;
ISAACS, NW ;
HAWTHORNTHWAITE, AM ;
HALLOREN, E ;
LINDSAY, JG .
JOURNAL OF MOLECULAR BIOLOGY, 1992, 224 (02) :527-528
[17]   PROJECTION STRUCTURE OF HALORHODOPSIN FROM HALOBACTERIUM-HALOBIUM AT 6-ANGSTROM RESOLUTION OBTAINED BY ELECTRON CRYOMICROSCOPY [J].
HAVELKA, WA ;
HENDERSON, R ;
HEYMANN, JAW ;
OESTERHELT, D .
JOURNAL OF MOLECULAR BIOLOGY, 1993, 234 (03) :837-846
[18]   STRUCTURE OF PURPLE MEMBRANE FROM HALOBACTERIUM-HALOBIUM - RECORDING, MEASUREMENT AND EVALUATION OF ELECTRON-MICROGRAPHS AT 3.5 A RESOLUTION [J].
HENDERSON, R ;
BALDWIN, JM ;
DOWNING, KH ;
LEPAULT, J ;
ZEMLIN, F .
ULTRAMICROSCOPY, 1986, 19 (02) :147-178
[19]  
JAY F, 1983, EUR J CELL BIOL, V30, P1
[20]   PIGMENT ORGANIZATION OF THE B800-850 ANTENNA COMPLEX OF RHODOPSEUDOMONAS-SPHAEROIDES [J].
KRAMER, HJM ;
VANGRONDELLE, R ;
HUNTER, CN ;
WESTERHUIS, WHJ ;
AMESZ, J .
BIOCHIMICA ET BIOPHYSICA ACTA, 1984, 765 (02) :156-165
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