INTERLEUKIN-2 MEDIATES P72(SYK) ACTIVATION IN PERIPHERAL-BLOOD LYMPHOCYTES

被引:17
作者
QIN, SF
INAZU, T
YANG, C
SADA, K
TANIGUCHI, T
YAMAMURA, H
机构
[1] FUKUI MED SCH,DEPT BIOCHEM,FUKUI 91011,JAPAN
[2] KOBE UNIV,BIOSIGNAL RES CTR,KOBE 657,JAPAN
关键词
INTERLEUKIN; 2; PROTEIN-TYROSINE KINASE P72(SYK); PERIPHERAL BLOOD LYMPHOCYTE;
D O I
10.1016/0014-5793(94)00450-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The ability of interleukin 2 (IL-2) to stimulate p72(syk) activity in intact porcine peripheral blood lymphocytes was examined. We demonstrated that IL-2 activated p72(syk) in a time- and dose-dependent manner, for which its peak time and maximum responsive dose were 5 min and 100 U/ml, respectively. This activation was observed only in cytosolic fractions and not in membrane ones. However, IL-2 failed to induce calcium mobilization. Moreover, IL-2-inducible p72(syk) activation was not affected when extra- and intracellular calcium was depleted. These data suggest that the IL-2 signaling pathway through p72(syk) in peripheral blood lymphocytes is different, at least in part, from other agonists, such as concanavalin A in polymorphonuclear neutrophils which can trigger both the activation of p72(syk) and intracellular calcium mobilization.
引用
收藏
页码:233 / 236
页数:4
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