AN ADAPTIVE, OBJECT-ORIENTED STRATEGY FOR BASE CALLING IN DNA-SEQUENCE ANALYSIS

被引：38

作者：

GIDDINGS, MC ^{[1
]}

BRUMLEY, RL ^{[1
]}

HAKER, M ^{[1
]}

SMITH, LM ^{[1
]}

机构：

[1] UNIV WISCONSIN,DEPT CHEM,1101 UNIV AVE,MADISON,WI 53706

来源：

NUCLEIC ACIDS RESEARCH | 1993年 / 21卷 / 19期

关键词：

D O I：

10.1093/nar/21.19.4530

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An algorithm has been developed for the determination of nucleotide sequence from data produced in fluorescence-based automated DNA sequencing instruments employing the four-color strategy. This algorithm takes advantage of object oriented programming techniques for modularity and extensibility. The algorithm is adaptive in that data sets from a wide variety of instruments and sequencing conditions can be used with good results. Confidence values are provided on the base calls as an estimate of accuracy. The algorithm iteratively employs confidence determinations from several different modules, each of which examines a different feature of the data for accurate peak identification. Modules within this system can be added or removed for increased performance or for application to a different task. In comparisons with commercial software, the algorithm performed well.

引用

页码：4530 / 4540

页数：11

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