PLASMID STABILITY IN BACILLUS-SPHAERICUS-2362 DURING RECYCLING IN MOSQUITO LARVAE

被引：6
作者：
SEYLER, RW [1 ]
YOUSTEN, AA [1 ]
BURKE, WF [1 ]
机构：
[1] ARIZONA STATE UNIV,DEPT MICROBIOL,TEMPE,AZ 85287
来源：
JOURNAL OF INVERTEBRATE PATHOLOGY | 1991年 / 58卷 / 03期
关键词：
BACILLUS-SPHAERICUS; CULEX-QUINQUEFASCIATUS; PLASMID STABILITY; PUB110; PLDT103; PLDT117; MICROBIAL PESTICIDE; BACTERIAL LARVICIDE RECYCLING; MOSQUITO CONTROL;
D O I：
10.1016/0022-2011(91)90181-O
中图分类号：
Q95 [动物学];
学科分类号：
071002 ;
摘要：
Bacillus sphaericus 2362 strains transformed with the plasmid pUB110 (4.5 kb) and plasmids derived from it, pLDT103 (7.6 kb) and pLDT117 (9.3 kb), were able to recycle (spore germination, vegetative growth, sporulation) in larvae of Culex quinquefasciatus. During the course of recycling, the pUB110 vector and the recombinant plasmid pLDT103 were stable (100 and 99.2%, respectively). However, the recombinant plasmid pLDT117 exhibited 23% segregational instability. Isolates which lost pLDT117 during recycling retained the one large plasmid native to B. sphaericus 2362. © 1991.
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页码：362 / 366
页数：5
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