AFFINITY CHROMATOGRAPHY OF CEREAL ALPHA-AMYLASE

被引：127

作者：

SILVANOVICH, MP ^{[1
]}

HILL, RD ^{[1
]}

机构：

[1] UNIV MANITOBA, DEPT PLANT SCI, WINNIPEG R3T 2N2, MANITOBA, CANADA

来源：

ANALYTICAL BIOCHEMISTRY | 1976年 / 73卷 / 02期

关键词：

D O I：

10.1016/0003-2697(76)90191-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An affinity chromatography technique for purification of .alpha.-amylase from triticale (X Triticosecale Wittmack) was described. Triticale .alpha.-amylase is retained on a column of cyclohepta-amylose epoxy Sepharose 6B. Contaminating proteins pass through in the sodium acetate elution buffer, but the .alpha.-amylase is selectively eluted with elution buffer containing cyclohepta-amylose. The chromatographic step yields in excess of 90% with a purification of up to 180-fold over crude extracts.

引用

页码：430 / 433

页数：4

共 7 条

[1]

Briggs D.E., 1961, Journal of the Institute of Brewing, V67, P427, DOI [10.1002/j.2050-0416.1961.tb01819.x, DOI 10.1002/J.2050-0416.1961.TB01819.X]

[2]

KRUGER JE, 1969, CEREAL CHEM, V46, P219

[3]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[4]

Loyter A., 1966, METHODS ENZYMOL, V8, P533, DOI [10.1016/0076-6879(66)08095-9, DOI 10.1016/0076-6879(66)08095-9]

[5]

MACGREGOR AW, 1971, CEREAL CHEM, V48, P490

[6] COMPETITIVE AFFINITY CHROMATOGRAPHY OF WHEAT ALPHA-AMYLASE [J].

TKACHUK, R .

FEBS LETTERS, 1975, 52 (01) :66-68

[7] IMMOBILIZATION OF LIGANDS FOR BIOSPECIFIC AFFINITY CHROMATOGRAPHY VIA THEIR HYDROXYL-GROUPS - CYCLOHEXAAMYLOSE-BETA-AMYLASE SYSTEM [J].

VRETBLAD, P .

FEBS LETTERS, 1974, 47 (01) :86-89

← 1 →