HEXOSAMINIDASE AND ALKALINE-PHOSPHATASE ACTIVITIES IN ARTICULAR CHONDROCYTES AND RELATIONSHIP TO CELL-CULTURE CONDITIONS

被引：3

作者：

MOKONJIMOBE, E ^{[1
]}

HECQUET, C ^{[1
]}

ROBIC, D ^{[1
]}

BOURBOUZE, R ^{[1
]}

ADOLPHE, M ^{[1
]}

机构：

[1] ECOLE PRAT HAUTES ETUD, CTR RECH BIOMED CORDELIERS, PHARM CELLULAIRE LAB, F-75006 PARIS, FRANCE

来源：

EXPERIENTIA | 1992年 / 48卷 / 04期

关键词：

HEXOSAMINIDASE; ALKALINE PHOSPHATASE; RABBIT; ARTICULAR CHONDROCYTES; CELL CULTURE CONDITIONS;

D O I：

10.1007/BF01923440

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Hexosaminidase and alkaline phosphatase activities in rabbit articular chondrocytes have been studied under different cell culture conditions. Chondrocytes were cultured in monolayer primary culture, monolayer subcultured to the fifth passage (in vitro aging) and cultured within a collagen gel; enzymatically released cartilage cells were used as control. Under these conditions, the two enzymes behave quite differently in relationship to alteration of the chondrocyte phenotype in culture. Increased lysosomal hexosaminidase activity could be considered to be a marker of the dedifferentiated phenotype in monolayer subculture; membrane alkaline phosphatase activity could be used as a marker of non-proliferating cells.

引用

页码：396 / 398

页数：3

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