BIOACTIVE POLYMERS .30. IMMOBILIZATION OF INVERTASE ON THE DIAZONIUM SALT OF 4-AMINOBENZOYLCELLULOSE

被引：21

作者：

SIMIONESCU, C

POPA, MI

DUMITRIU, S

机构：

[1] Polytechnic Institute of Jassy, Department of Organic and Macromolecular Chemistry, R 6600 Jassy, Romania

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1987年 / 29卷 / 03期

关键词：

CELLULOSE DERIVATIVES - HYDROGEN ION CONCENTRATION;

D O I：

10.1002/bit.260290312

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Invertase was immobilized on diazotized 4-aminobenzoylcellulose. The optimum coupling conditions, namely enzyme concentration, time, and pH, were determined. The temperature and pH value for the maximum activity of the immobilized enzyme were determined and the apparent Michaelis constant was estimated.

引用

页码：361 / 365

页数：5

共 11 条

[1]

COCHRAN WG, 1968, EXPT DESIGNS, P335

[2]

Dinelli D, 1976, Methods Enzymol, V44, P227

[3]

DUMITRIU S, 1980, REV ROUM CHIM, V25, P989

[4]

DUMITRIU S, 1968, CULLULOSE CHEM TECHN, V2, P321

[5] PREPARATION OF IMMOBILIZED INVERTASE USING POLYVINYL-ALCOHOL) MEMBRANE [J].

IMAI, K ;

SHIOMI, T ;

SATO, K ;

FUJISHIMA, A .

BIOTECHNOLOGY AND BIOENGINEERING, 1983, 25 (02) :613-617

[6]

KENT C, 1978, TOPICS ENZYME FERMEN, V2, P276

[7]

LOPEZSANTIN J, 1983, J CHEM TECH BIOT B, V33, P101

[8]

Mosbach K, 1976, Methods Enzymol, V44, P453

[9] BIOACTIVE POLYMERS .15. HYDROLASES IMMOBILIZED ON BIOZAN-R [J].

SIMIONESCU, C ;

DUMITRIU, S ;

POPA, M ;

DUMITRIU, M ;

HRITCU, D .

COLLOID AND POLYMER SCIENCE, 1984, 262 (09) :705-711

[10]

SIMIONESCU CI, 1984, POLYM BULL, V12, P369

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