ISOLATION AND PURIFICATION OF HS-142-1, A NOVEL NONPEPTIDE ANTAGONIST FOR THE ATRIAL-NATRIURETIC-PEPTIDE RECEPTOR, FROM AUREOBASIDIUM SP

被引：31

作者：

MORISHITA, Y ^{[1
]}

TAKAHASHI, M ^{[1
]}

SANO, T ^{[1
]}

KAWAMOTO, I ^{[1
]}

ANDO, K ^{[1
]}

SANO, H ^{[1
]}

SAITOH, Y ^{[1
]}

KASE, H ^{[1
]}

MATSUDA, Y ^{[1
]}

机构：

[1] KYOWA HAKKO KOGYO CO LTD, TOKYO RES LABS,3-6-6 ASAHIMACHI, MACHIDA, TOKYO 194, JAPAN

来源：

AGRICULTURAL AND BIOLOGICAL CHEMISTRY | 1991年 / 55卷 / 12期

关键词：

D O I：

10.1080/00021369.1991.10857920

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

During the screening program for atrial natriuretic peptide (ANP) receptor ligands of microbial origin, we isolated a novel nonpeptide ANP antagonist, HS-142-1, from a culture broth of Aureobasidium pullulans var. melanigenum. Structural analysis showed that HS-142-1 was composed of 20-30 kinds of beta-1,6-glucan esterified by caproyl groups; each component had an almost equal potency. HS-142-1 inhibited [I-125]-rANP binding to its receptor in rabbit kidney cortex membranes with an IC50 of 0.3-mu-g/ml and antagonized ANP-induced cGMP production by bovine lung membranes in a dose-dependent fashion. The discovery of this nonpeptide ANP antagonist, HS-142-1, will provide a useful tool to study the physiological significance of natriuretic peptide system.

引用

页码：3017 / 3025

页数：9

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