STRUCTURE OF 2 SULFATED OLIGOSACCHARIDES FROM RESPIRATORY MUCINS OF A PATIENT SUFFERING FROM CYSTIC-FIBROSIS - A FAST-ATOM-BOMBARDMENT MS AND H-1-NMR SPECTROSCOPIC STUDY

Human respiratory-mucin glycopeptides were isolated from the sputum of a patient suffering from cystic fibrosis. They were subjected to treatment with alkaline borohydride. Application of ion-exchange chromatography afforded carbohydrate fractions containing sulphate. One of these fractions containing a mixture of sulphated oligosaccharides was subsequently submitted to gel-filtration chromatography and h.p.l.c. Two sulphated oligosaccharide-alditols, A and B, were prepared; their structure was determined by means of 400 MHz H-1-n.m.r. spectroscopy and fast-atombombardment m.s. They had a core type 2 and the sulphate was 3-linked to a terminal galactose residue: GRAPHICS