COMPUTATIONAL TOOLS FOR RATIONAL PROTEIN ENGINEERING OF ALDOLASES

被引：5
作者：
Widmann, Michael [1 ]
Pleiss, Juergen [1 ]
Samland, Anne K. [2 ]
机构：
[1] Univ Stuttgart, Inst Tech Biochem, D-70569 Stuttgart, Germany
[2] Univ Stuttgart, Inst Microbiol, Allmandring 31, D-70569 Stuttgart, Germany
来源：
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL | 2012年 / 2卷 / 03期
关键词：
protein engineering; substrate specificity; stereoselectivity; de novo design; transaldolase; biocatalysis;
D O I：
10.5936/csbj.201209016
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In this mini-review we describe the different strategies for rational protein engineering and summarize the computational tools available. Computational tools can either be used to design focused libraries, to predict sequence-function relationships or for structure-based molecular modelling. This also includes de novo design of enzymes. Examples for protein engineering of aldolases and transaldolases are given in the second part of the mini-review.
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页数：11
相关论文
共 105 条
[71]   Iterative saturation mutagenesis on the basis of B factors as a strategy for increasing protein thermostability [J].
Reetz, Manfred T. ;
D Carballeira, Jose ;
Vogel, Andreas .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (46) :7745-7751
[72]   Directed evolution of enantioselective enzymes: Iterative cycles of CASTing for probing protein-sequence space [J].
Reetz, MT ;
Wang, LW ;
Bocola, M .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (08) :1236-1241
[73]   Expanding the range of substrate acceptance of enzymes: Combinatorial active-site saturation test [J].
Reetz, MT ;
Bocola, M ;
Carballeira, JD ;
Zha, DX ;
Vogel, A .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2005, 44 (27) :4192-4196
[74]  
Reetz MT, 2001, ANGEW CHEM INT EDIT, V40, P3589, DOI 10.1002/1521-3773(20011001)40:19<3589::AID-ANIE3589>3.0.CO
[75]  
2-X
[76]   Modeling structurally variable regions in homologous proteins with rosetta [J].
Rohl, CA ;
Strauss, CEM ;
Chivian, D ;
Baker, D .
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 2004, 55 (03) :656-677
[77]   Structurally Informed Site-Directed Mutagenesis of a Stereochemically Promiscuous Aldolase To Afford Stereochemically Complementary Biocatalysts [J].
Royer, Sylvain F. ;
Haslett, Luke ;
Crennell, Susan J. ;
Hough, David W. ;
Danson, Michael J. ;
Bull, Steven D. .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2010, 132 (33) :11753-11758
[78]   The Influence of Protein Dynamics on the Success of Computational Enzyme Design [J].
Ruscio, Jory Z. ;
Kohn, Jonathan E. ;
Ball, K. Aurelia ;
Head-Gordon, Teresa .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2009, 131 (39) :14111-14115
[79]   Microbial aldolases as C-C bonding enzymes - unknown treasures and new developments [J].
Samland, Anne K. ;
Sprenger, Georg A. .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2006, 71 (03) :253-264
[80]   The Transaldolase Family: New Synthetic Opportunities from an Ancient Enzyme Scaffold [J].
Samland, Anne K. ;
Rale, Madhura ;
Sprenger, Georg A. ;
Fessner, Wolf-Dieter .
CHEMBIOCHEM, 2011, 12 (10) :1454-1474
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