COMPUTATIONAL TOOLS FOR RATIONAL PROTEIN ENGINEERING OF ALDOLASES

被引：5
作者：
Widmann, Michael [1 ]
Pleiss, Juergen [1 ]
Samland, Anne K. [2 ]
机构：
[1] Univ Stuttgart, Inst Tech Biochem, D-70569 Stuttgart, Germany
[2] Univ Stuttgart, Inst Microbiol, Allmandring 31, D-70569 Stuttgart, Germany
来源：
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL | 2012年 / 2卷 / 03期
关键词：
protein engineering; substrate specificity; stereoselectivity; de novo design; transaldolase; biocatalysis;
D O I：
10.5936/csbj.201209016
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In this mini-review we describe the different strategies for rational protein engineering and summarize the computational tools available. Computational tools can either be used to design focused libraries, to predict sequence-function relationships or for structure-based molecular modelling. This also includes de novo design of enzymes. Examples for protein engineering of aldolases and transaldolases are given in the second part of the mini-review.
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页数：11
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