COMPUTATIONAL TOOLS FOR RATIONAL PROTEIN ENGINEERING OF ALDOLASES

被引：5

作者：

Widmann, Michael ^{[1
]}

Pleiss, Juergen ^{[1
]}

Samland, Anne K. ^{[2
]}

机构：

[1] Univ Stuttgart, Inst Tech Biochem, D-70569 Stuttgart, Germany

[2] Univ Stuttgart, Inst Microbiol, Allmandring 31, D-70569 Stuttgart, Germany

来源：

COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL | 2012年 / 2卷 / 03期

关键词：

protein engineering; substrate specificity; stereoselectivity; de novo design; transaldolase; biocatalysis;

D O I：

10.5936/csbj.201209016

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this mini-review we describe the different strategies for rational protein engineering and summarize the computational tools available. Computational tools can either be used to design focused libraries, to predict sequence-function relationships or for structure-based molecular modelling. This also includes de novo design of enzymes. Examples for protein engineering of aldolases and transaldolases are given in the second part of the mini-review.

引用

页数：11

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