TRANSGENIC MICE CONTAINING A HUMAN HEAVY-CHAIN IMMUNOGLOBULIN GENE FRAGMENT CLONED IN A YEAST ARTIFICIAL CHROMOSOME

被引：77
作者：
CHOI, TK
HOLLENBACH, PW
PEARSON, BE
UEDA, RM
WEDDELL, GN
KURAHARA, CG
WOODHOUSE, CS
KAY, RM
LORING, JF
机构：
[1] GenPharm International, Mountain View, CA, 94043
来源：
NATURE GENETICS | 1993年 / 4卷 / 02期
关键词：
D O I：
10.1038/ng0693-117
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
We have developed a method for the introduction of yeast artificial chromosomes (YACs) into transgenic mice. An 85 kilobase (kb) fragment of the human heavy chain immunoglobulin gene was cloned as a YAC, and embryonic stem cell lines carrying intact, integrated YACs were derived by co-lipofection of the YAC with an unlinked selectable marker. Chimaeric founder animals were produced by blastocyst injection, and offspring transgenic for the YAC were obtained. Analysis of serum from these offspring for human heavy chain antibody subunits demonstrated expression of the YAC-borne immunoglobulin gene fragment. Co-lipofection may prove to be a highly-successful means of producing transgenic mice containing large gene fragments in YACs.
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收藏
页码：117 / 123
页数：7
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