STUDIES ON SPERM CHROMATIN STRUCTURE ALTERATIONS AND CYTOGENETIC DAMAGE OF MOUSE SPERM FOLLOWING IN-VITRO INCUBATION - STUDIES ON IN-VITRO-INCUBATED MOUSE SPERM

被引：0
作者：
ESTOP, AM
MUNNE, S
JOST, LK
EVENSON, DP
机构：
[1] S DAKOTA STATE UNIV,DEPT CHEM,BROOKINGS,SD 57007
[2] UNIV PITTSBURGH,DEPT HUMAN GENET,PITTSBURGH,PA 15260
来源：
JOURNAL OF ANDROLOGY | 1993年 / 14卷 / 04期
关键词：
SPERM AGING; IN-VITRO FERTILIZATION; CHROMATIN CHROMOSOME STRUCTURE; DNA DENATURATION; FLOW CYTOMETRY;
D O I：
暂无
中图分类号：
R69 [泌尿科学（泌尿生殖系疾病）];
学科分类号：
摘要：
Mouse epididymal sperm incubated in Tyrode's T6 fertilization media were analyzed over time for chromosome damage by two methods. First, cytogenetic analysis was done on paternal pronuclei metaphase chromosomes. After 6 hours incubation 11% of the cells demonstrated chromosome structural abnormalities. Secondly, sperm nuclei were measured by the sperm chromatin structure assay, which is a measure of the susceptibility of sperm DNA to the nuclei demonstrated an increased susceptibility to DNA denaturation, reaching near 100% by 48 hours. Changes in chromatin structure at the molecular level may lead to chromosome breaks seen in-pronuclear chromosomes.
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页码：282 / 288
页数：7
相关论文
共 32 条
[1]  
ALBANESE R, 1982, MUTAT RES, V2, P315
[2]   EFFECT OF THE DEGREE OF MATURATION OF MOUSE OOCYTES AT FERTILIZATION - A SOURCE OF CHROMOSOME IMBALANCE [J].
BADENAS, J ;
SANTALO, J ;
CALAFELL, JM ;
ESTOP, AM ;
EGOZCUE, J .
GAMETE RESEARCH, 1989, 24 (02) :205-218
[3]   HETEROGENEITY OF SPERM NUCLEAR CHROMATIN STRUCTURE AND ITS RELATIONSHIP TO BULL FERTILITY [J].
BALLACHEY, BE ;
HOHENBOKEN, WD ;
EVENSON, DP .
BIOLOGY OF REPRODUCTION, 1987, 36 (04) :915-925
[4]  
CHAN YM, 1990, FERTIL STERIL, V54, P730
[5]   LYMPHOCYTE STIMULATION - RAPID MULTIPARAMETER ANALYSIS [J].
DARZYNKIEWICZ, Z ;
TRAGANOS, F ;
SHARPLESS, T ;
MELAMED, MR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (08) :2881-2884
[6]   MUTAGENICITY TESTING OF VINCRISTINE SULFATE IN GERM-CELLS OF MALE-MICE [J].
EHLING, UH ;
KRATOCHVILOVA, J ;
LEHMACHER, W ;
NEUHAUSERKLAUS, A .
MUTATION RESEARCH, 1988, 209 (3-4) :107-113
[7]  
EHLING UH, 1988, MUTAT RES, V199, P191
[8]  
Evenson D.P., 1989, FLOW CYTOMETRY ADV R, VI, P217
[9]   FLOW CYTOMETRIC ANALYSIS OF MOUSE SPERMATOGENIC FUNCTION FOLLOWING EXPOSURE TO ETHYLNITROSOUREA [J].
EVENSON, DP ;
HIGGINS, PJ ;
GRUENEBERG, D ;
BALLACHEY, BE .
CYTOMETRY, 1985, 6 (03) :238-253
[10]   TOXICITY OF THIOTEPA ON MOUSE SPERMATOGENESIS AS DETERMINED BY DUAL-PARAMETER FLOW-CYTOMETRY [J].
EVENSON, DP ;
BAER, RK ;
JOST, LK ;
GESCH, RW .
TOXICOLOGY AND APPLIED PHARMACOLOGY, 1986, 82 (01) :151-163
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