FERRITINS OF SCHISTOSOMA-MANSONI - SEQUENCE COMPARISON AND EXPRESSION IN FEMALE AND MALE WORMS

被引:57
作者
DIETZEL, J [1 ]
HIRZMANN, J [1 ]
PREIS, D [1 ]
SYMMONS, P [1 ]
KUNZ, W [1 ]
机构
[1] UNIV DUSSELDORF,INST GENET,UNIV STR 1,W-4000 DUSSELDORF 1,GERMANY
关键词
FERRITIN GENE SEQUENCE; FERRITIN GENE EXPRESSION; SCHISTOSOMA; SEX SPECIFICITY;
D O I
10.1016/0166-6851(92)90221-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Recombinant clones of Schistosoma mansoni cDNA libraries containing the complete coding regions of 2 different ferritin subunits have been isolated and sequenced. This allows for the first time a comparison of ferritin sequences from an invertebrate with those of vertebrates. The deduced amino acid sequences of both Schistosoma ferritin subunit clones show significant homology to vertebrate ferritin H chains. Similarity exceeds 50% identity and includes the recently identified ferroxidase center which is present only in H chains. However, non-conservative substitutions of amino acid residues lining the 3-fold symmetry channel were found, and a gap of 3 successive amino acids unique to the 2 Schistosoma ferritin sequences was identified. Remarkably, for each of the 2 genes, we found a conspicuous difference in the amount of ferritin transcripts between females and males: one of the genes is preferentially expressed in females, the other in males.
引用
收藏
页码:245 / 254
页数:10
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