H+/E- STOICHIOMETRY OF MITOCHONDRIAL CYTOCHROME COMPLEXES RECONSTITUTED IN LIPOSOMES - RATE-DEPENDENT CHANGES OF THE STOICHIOMETRY IN THE CYTOCHROME-C-OXIDASE VESICLES

被引：45

作者：

CAPITANIO, N ^{[1
]}

CAPITANIO, G ^{[1
]}

DENITTO, E ^{[1
]}

VILLANI, G ^{[1
]}

PAPA, S ^{[1
]}

机构：

[1] UNIV BARI,FAC MED,INST MED BIOCHEM & CHEM,PIAZZA GIULIO CESARE,I-70124 BARI,ITALY

来源：

FEBS LETTERS | 1991年 / 288卷 / 1-2期

关键词：

CYTOCHROME-C OXIDASE; CYTOCHROME-C REDUCTASE; H+ PUMPING; H+/E- STOICHIOMETRY; PROTEOLIPOSOME;

D O I：

10.1016/0014-5793(91)81029-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The H+/e- stoichiometry of protonmotive cytochrome c oxidase, isolated from bovine heart mitochondria and reconstituted in liposomes, has been determined by making use of direct spectrophotometric measurements of the initial rates of e- flow and H+ translocation. It is shown that the <-- H+/e- ratio for redox-linked proton ejection by the oxidase varies from around 0 to a maximum of 1 as a function of the rate of overall electron flow in the complex.

引用

页码：179 / 182

页数：4

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