MOLECULAR-CLONING AND SEQUENCE-ANALYSIS OF HAMSTER CYP2E1

被引：8
|
作者：
SAKUMA, T [1 ]
TAKAI, M [1 ]
YOKOI, T [1 ]
KAMATAKI, T [1 ]
机构：
[1] HOKKAIDO UNIV,FAC PHARMACEUT SCI,DIV DRUG METAB,SAPPORO,HOKKAIDO 060,JAPAN
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1994年 / 1217卷 / 02期
关键词：
CYTOCHROME P-450; CLONING; 2E GENE SUBFAMILY; (SYRIAN HAMSTER); (LIVER);
D O I：
10.1016/0167-4781(94)90043-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Two cDNA clones, pHSj31 and pHSj3, coding for CYP2E1 were isolated from a hamster liver cDNA library. The sequence analyses revealed that they encoded the same polypeptide of 493 amino acid residues (M(r) = 56 616) and differed from the length of their 3'-untranslated region. The deduced amino acid sequence of hamster CYP2E1 showed approx. 90% identities with those of the rats and mice, and approx. 80% identities with those of the rabbits, monkeys and humans. The NH2-terminal 35 deduced amino acid sequences of hamster CYP2E1 were completely identical with purified protein, ha P-450j. The Northern blot analysis showed that CYP2E1 was expressed in livers and to lesser extents in kidneys and lungs.
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收藏
页码：229 / 231
页数：3
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