INORGANIC PYROPHOSPHATASE OF CLOFIBRATE-INDUCED RAT-LIVER PEROXISOMES

被引：5

作者：

SHIMIZU, S ^{[1
]}

OHKUMA, S ^{[1
]}

机构：

[1] KANAZAWA UNIV,FAC PHARMACEUT SCI,DEPT BIOCHEM,13-1 TAKARA MACHI,KANAZAWA,ISHIKAWA 920,JAPAN

来源：

JOURNAL OF BIOCHEMISTRY | 1993年 / 113卷 / 04期

关键词：

D O I：

10.1093/oxfordjournals.jbchem.a124067

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Clofibrate increased inorganic pyrophosphatase (PPase) activity in peroxisomes more than 12-fold (740 milliunits/head, 15.9 +/- 5.0 milliunits/mg protein) in rat liver. The distribution of cytochrome c oxidase and that of the PPase in a Nyeodenz gradient suggested that the PPase is an original peroxisomal enzyme and not a mitochondrial contaminant: This was confirmed by second Nycodenz gradient centrifugation. The optimum pH of the peroxisomal PPase was about 8.5. The activity was specific to inorganic pyrophosphate (PP(i)), the K(m) value for PP(i) being 34.1 +/- 3.3 muM. It was strictly dependent on Mg2+ and showed a sigmoidal dose-response for Mg2+ with an S0.5 value of 100 muM. The activity was inhibited by Ca2+, p-chloromercuriphenylsulfonic acid, Hg2+, N-ethylmaleimide, and NaF. The functions of peroxisomal PPase are discussed.

引用

页码：462 / 466

页数：5

共 29 条

[21] PEROXISOMAL LOCALIZATION OF GLUCOSE-6-PHOSPHATE-DEHYDROGENASE AND PYROPHOSPHATE-STIMULATED DIHYDROXYACETONE-PHOSPHATE ACYLTRANSFERASE IN MOUSE KIDNEY [J].

PATEL, BN ;

MACKNESS, MI ;

CONNOCK, MJ .

BIOCHEMICAL JOURNAL, 1987, 244 (02) :443-448

[22] TISSUE FRACTIONATION STUDIES .15. INTRACELLULAR DISTRIBUTION AND PROPERTIES OF BETA-N-ACETYLGLUCOSAMINIDASE AND BETA-GALACTOSIDASE IN RAT LIVER [J].

SELLINGER, OZ ;

BEAUFAY, H ;

JACQUES, P ;

DOYEN, A ;

DEDUVE, C .

BIOCHEMICAL JOURNAL, 1960, 74 :450-456

[23] INDUCTION AND CHARACTERIZATION OF 2 TYPES OF ATPASE ON RAT-LIVER PEROXISOMES [J].

SHIMIZU, S ;

IMANAKA, T ;

TAKANO, T ;

OHKUMA, S .

JOURNAL OF BIOCHEMISTRY, 1992, 112 (03) :376-384

[24] MAJOR ATPASES ON CLOFIBRATE-INDUCED RAT-LIVER PEROXISOMES ARE NOT ASSOCIATED WITH 70-KDA PEROXISOMAL MEMBRANE-PROTEIN (PMP70) [J].

SHIMIZU, S ;

IMANAKA, T ;

TAKANO, T ;

OHKUMA, S .

JOURNAL OF BIOCHEMISTRY, 1992, 112 (06) :733-736

[25]

SINGH I, 1992, J BIOL CHEM, V267, P13306

[26]

VANVELDHOVEN PP, 1987, J BIOL CHEM, V262, P4310

[27] ISOLATION AND SUBUNIT COMPOSITION OF MEMBRANE INORGANIC PYROPHOSPHATASE FROM RAT-LIVER MITOCHONDRIA [J].

VOLK, SE ;

BAYKOV, AA .

BIOCHIMICA ET BIOPHYSICA ACTA, 1984, 791 (02) :198-204

[28] ISOLATION, SUBUNIT STRUCTURE AND LOCALIZATION OF INORGANIC PYROPHOSPHATASE OF HEART AND LIVER-MITOCHONDRIA [J].

VOLK, SE ;

BAYKOV, AA ;

KOSTENKO, EB ;

AVAEVA, SM .

BIOCHIMICA ET BIOPHYSICA ACTA, 1983, 744 (02) :127-134

[29]

YOSHIDA C, 1982, CANCER RES, V42, P3526

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