DISTRIBUTION OF CHARGE ON PHOTORECEPTOR DISK MEMBRANES AND IMPLICATIONS FOR CHARGED LIPID ASYMMETRY

被引：40
|
作者：
TSUI, FC [1 ]
SUNDBERG, SA [1 ]
HUBBELL, WL [1 ]
机构：
[1] UNIV CALIF LOS ANGELES,DEPT CHEM & BIOCHEM,LOS ANGELES,CA 90024
来源：
BIOPHYSICAL JOURNAL | 1990年 / 57卷 / 01期
关键词：
D O I：
10.1016/S0006-3495(90)82509-1
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
A novel spin labeling technique is used to determine both the inner and outer surface potentials of isolated rod outer segment disc membranes and of reconstituted membranes containing rhodopsin with defined lipid compositions. It is shown that these potentials can be accounted for in a consistent manner by the accepted model of rhodopsin, the known lipid composition, and the Gouy-Chapman theory, provided the charged lipid is asymmetric in the membrane, with approximately 75% on the external surface. © 1990, The Biophysical Society. All rights reserved.
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页码：85 / 97
页数：13
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