ENANTIOMERIC SEPARATION OF AMPHETAMINE RELATED DRUGS BY CAPILLARY ZONE ELECTROPHORESIS USING NATIVE AND DERIVATIZED BETA-CYCLODEXTRIN AS CHIRAL ADDITIVES

Amphetamine, methamphetamine and several ring-substituted analogs which are under governmental regulations have been separated by capillary zone electrophoresis employing native and various substituted beta-cyclodextrins as additives to the background electrolyte. The following chiral selectors were used: native beta-cyclodextrin, heptakis-(2,6-di-O-methyl)-beta-cyclodextrin, heptakis-(2,3,6-tri-O-methyl)-beta-cyclodextrin, (2-hydroxy)propyl-beta-cyclodextrin and carboxymethyl-beta-cyclodextrin. The amphetamines were separated without derivatization. Separations are reported with respect to the kind of chiral selector. Native beta-cyclodextrin and carboxymethyl-beta-cyclodextrin turned out to give optimal resolutions within only a few minutes. This direct method is compared with the indirect method separating the diastereomeric Marfey's derivatized amphetamines by means of non-chiral sodium dodecylsulfate micelles.