IMMUNOGOLD LABELING OF ESTRADIOL-RECEPTOR IN MCF7 CELLS

被引:17
作者
SIERRALTA, WD
BONIG, I
THOLE, HH
机构
关键词
ESTRADIOL RECEPTOR; BREAST CANCER CELLS; CELL CULTURE; ULTRASTRUCTURE; ELECTRON MICROSCOPY; IMMUNOHISTOCHEMISTRY; HUMAN;
D O I
10.1007/s004410050303
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The distribution of estradiol receptor in serial sections of estradiol-deprived and estradiol-stimulated MCF7 cells was studied by using mouse monoclonal antibodies reacting with different domains of the receptor and goat-antimouse IgG/6 nm gold. In the nucleus and the cytoplasm of estradiol-deprived cells, the receptor was detected by all three monoclonals (13H2, HT 65 and MA1-310). The antibodies 13H2 and MA1-310 detected receptor associated to the microfilament bundles in the cytoplasm. Higher densities of antireceptor attachment to the nuclear areas were accompanied by a reduction in the attachment to the cytoplasm after estradiol stimulation of the cells. The results confirm earlier observations on the presence of cytoplasmic estrogen receptor in estradiol-deprived cells and support the premise of an estradiol-induced translocation of this ligand-dependent transcription regulator.
引用
收藏
页码:445 / 452
页数:8
相关论文
共 27 条
  • [1] AKNER G, 1990, EUR J CELL BIOL, V53, P390
  • [2] BARSONY J, 1992, J BIOL CHEM, V267, P24457
  • [3] ESTROGEN-RECEPTORS IN DENDRITES AND AXON TERMINALS IN THE GUINEA-PIG HYPOTHALAMUS
    BLAUSTEIN, JD
    LEHMAN, MN
    TURCOTTE, JC
    GREENE, G
    [J]. ENDOCRINOLOGY, 1992, 131 (01) : 281 - 290
  • [4] REGULATION OF PROGESTERONE-RECEPTOR GENE-EXPRESSION IN MCF-7 BREAST-CANCER CELLS - A COMPARISON OF THE EFFECTS OF CYCLIC ADENOSINE-3',5'-MONOPHOSPHATE, ESTRADIOL, INSULIN-LIKE GROWTH FACTOR-I, AND SERUM FACTORS
    CHO, HS
    ARONICA, SM
    KATZENELLENBOGEN, BS
    [J]. ENDOCRINOLOGY, 1994, 134 (02) : 658 - 664
  • [5] ENGEL L, 1978, CANCER RES, V38, P4328
  • [6] HUMAN ESTROGEN-RECEPTOR CDNA - SEQUENCE, EXPRESSION AND HOMOLOGY TO V-ERB-A
    GREEN, S
    WALTER, P
    KUMAR, V
    KRUST, A
    BORNERT, JM
    ARGOS, P
    CHAMBON, P
    [J]. NATURE, 1986, 320 (6058) : 134 - 139
  • [7] NUCLEOCYTOPLASMIC SHUTTLING OF THE PROGESTERONE-RECEPTOR
    GUIOCHONMANTEL, A
    LESCOP, P
    CHRISTINMAITRE, S
    LOOSFELT, H
    PERROTAPPLANAT, M
    MILGROM, E
    [J]. EMBO JOURNAL, 1991, 10 (12) : 3851 - 3859
  • [8] A 2-STEP MECHANISM FOR INTERACTION OF ESTRADIOL WITH RAT UTERUS
    JENSEN, EV
    SUZUKI, T
    KAWASHIMA, T
    STUMPF, WE
    JUNGBLUT, PW
    DESOMBRE, ER
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1968, 59 (02) : 632 - +
  • [9] JENSEN EV, 1991, NUCLEAR HORMONE RECE, P1
  • [10] MONOCLONAL-ANTIBODIES LOCALIZE ESTROGEN-RECEPTOR IN THE NUCLEI OF TARGET-CELLS
    KING, WJ
    GREENE, GL
    [J]. NATURE, 1984, 307 (5953) : 745 - 747