FILLING THE GAPS IN THE PORCINE LINKAGE MAP - ISOLATION OF MICROSATELLITES FROM CHROMOSOME-18 USING FLOW SORTING AND SINE-PCR

被引：7
作者：
ELLEGREN, H
BASU, T
机构：
[1] Dept. Animal Breeding and Genetics, Swedish Univ. of Agric. Sciences, S-751 24 Uppsala
来源：
CYTOGENETICS AND CELL GENETICS | 1995年 / 71卷 / 04期
关键词：
D O I：
10.1159/000134141
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Flow-sorted chromosome 18 material from the pig (SSC18) was amplified with SINE-PCR to generate DNA for molecular cloning. An SSC18-enriched library was constructed and subsequently screened with a (CA)IS probe to identify polymorphic microsatellites. Eleven unique microsatellites were obtained, six of which constituted direct extensions of SINE poly(A) tracts. Eight primer pairs amplified polymorphic loci in unrelated pigs. The polymorphic markers were typed in a Swedish reference pedigree for porcine genome mapping, but only two of them (S0177 and S0179) mapped to SSC18. The remaining markers mapped to chromosomes 3, 4, 7, 9, and 11. Together with two markers from other sources (S0062 and sw787), a four-point linkage group on SSC18 could be established.
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收藏
页码：370 / 373
页数：4
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