THE STRETCH-ACTIVATED ION-CHANNEL BLOCKER GADOLINIUM ALSO BLOCKS L-TYPE CALCIUM CHANNELS IN ISOLATED VENTRICULAR MYOCYTES OF THE GUINEA-PIG

被引：145

作者：

LACAMPAGNE, A ^{[1
]}

GANNIER, F ^{[1
]}

ARGIBAY, J ^{[1
]}

GARNIER, D ^{[1
]}

LEGUENNEC, JY ^{[1
]}

机构：

[1] FAC SCI TOURS, PHYSIOL CELLULES CARD & VASC LAB, CNRS, EP21, F-37200 TOURS, FRANCE

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1994年 / 1191卷 / 01期

关键词：

GADOLINIUM; CADMIUM; CALCIUM CURRENT; STRETCH-ACTIVATED CHANNEL;

D O I：

10.1016/0005-2736(94)90250-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We show that gadolinium (Gd3(+)) is a potent calcium channel blocker in guinea-pig isolated ventricular myocytes. A dose-dependent inhibition of I-CaL. was found with an EC(50) of 1.4 mu M and a complete inhibition at 10 mu M Gd3(+). When compared with Cd2+, it appeared that the blockade of I-CaL. is a complex phenomenon probably involving more than one sitphenomenon probably involving more than one site of interaction (a Hill coefficient of 1.6 was found for Gd3+ vs. 1.0 for Cd2+). It is concluded that Gd3+ ions completely block I-CaL. at concentrations used to block stretch-activated channels (SAC), rendering its use as a specific SAC inhibitor problematic.

引用

页码：205 / 208

页数：4

共 16 条

[1] GADOLINIUM BLOCKS LOW-THRESHOLD AND HIGH-THRESHOLD CALCIUM CURRENTS IN PITUITARY-CELLS [J].

BIAGI, BA ;

ENYEART, JJ .

AMERICAN JOURNAL OF PHYSIOLOGY, 1990, 259 (03) :C515-C520

[2] MODULATION OF CALCIUM CHANNELS IN CARDIAC AND NEURONAL CELLS BY AN ENDOGENOUS PEPTIDE [J].

CALLEWAERT, G ;

HANBAUER, I ;

MORAD, M .

SCIENCE, 1989, 243 (4891) :663-666

[3] STRETCH-INDUCED INCREASES IN INTRACELLULAR CALCIUM OF ISOLATED VASCULAR SMOOTH-MUSCLE CELLS [J].

DAVIS, MJ ;

MEININGER, GA ;

ZAWIEJA, DC .

AMERICAN JOURNAL OF PHYSIOLOGY, 1992, 263 (04) :H1292-H1299

[4] GADOLINIUM SELECTIVELY BLOCKS A COMPONENT OF CALCIUM CURRENT IN RODENT NEURO-BLASTOMA X GLIOMA HYBRID (NG108-15) CELLS [J].

DOCHERTY, RJ .

JOURNAL OF PHYSIOLOGY-LONDON, 1988, 398 :33-47

[5] TISSUE-SPECIFIC EXPRESSION OF CALCIUM CHANNELS [J].

HULLIN, R ;

BIEL, M ;

FLOCKERZI, V ;

HOFMANN, F .

TRENDS IN CARDIOVASCULAR MEDICINE, 1993, 3 (02) :48-53

[6] A NEW METHOD OF ATTACHMENT OF ISOLATED MAMMALIAN VENTRICULAR MYOCYTES FOR TENSION RECORDING - LENGTH DEPENDENCE OF PASSIVE AND ACTIVE TENSION [J].

LEGUENNEC, JY ;

PEINEAU, N ;

ARGIBAY, JA ;

MONGO, KG ;

GARNIER, D .

JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 1990, 22 (10) :1083-1093

[7] STRETCH-INDUCED INCREASE OF RESTING INTRACELLULAR CALCIUM-CONCENTRATION IN SINGLE GUINEA-PIG VENTRICULAR MYOCYTES [J].

LEGUENNEC, JY ;

WHITE, E ;

GANNIER, F ;

ARGIBAY, JA ;

GARNIER, D .

EXPERIMENTAL PHYSIOLOGY, 1991, 76 (06) :975-978

[8] INVOLVEMENT OF STRETCH-ACTIVATED ION CHANNELS IN CA-2+ MOBILIZATION TO MECHANICAL STRETCH IN ENDOTHELIAL-CELLS [J].

NARUSE, K ;

SOKABE, M .

AMERICAN JOURNAL OF PHYSIOLOGY, 1993, 264 (04) :C1037-C1044

[9]

PELZER D, 1990, REV PHYSIOL BIOCH P, V114, P107

[10] ROLES OF MECHANOSENSITIVE ION CHANNELS, CYTOSKELETON, AND CONTRACTILE ACTIVITY IN STRETCH-INDUCED IMMEDIATE-EARLY GENE-EXPRESSION AND HYPERTROPHY OF CARDIAC MYOCYTES [J].

SADOSHIMA, J ;

TAKAHASHI, T ;

JAHN, L ;

IZUMO, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (20) :9905-9909

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