INSITU AND SLOT HYBRIDIZATION ANALYSIS OF RNA IN COLORECTAL TUMORS AND NORMAL COLON SHOWS DISTINCT DISTRIBUTIONS OF MITOCHONDRIAL SEQUENCES

被引：13

作者：

CHESTER, KA

ROBSON, L

BEGENT, RHJ

PRINGLE, H

PRIMROSE, L

TALBOT, IC

MACPHERSON, AJS

OWEN, SL

BOXER, G

MALCOLM, ADB

机构：

[1] CHARING CROSS & WESTMINSTER MED SCH,CANC RES CAMPAIGN LABS,LONDON W6 8RP,ENGLAND

[2] LEICESTER ROYAL INFIRM,DEPT PATHOL,LEICESTER LE2 7XL,ENGLAND

[3] ST MARKS HOSP,DEPT PATHOL,LONDON EC1V 2PS,ENGLAND

[4] IMPERIAL CANC RES FUND,COLORECTAL CANC UNIT,LONDON EC1V 2PS,ENGLAND

来源：

JOURNAL OF PATHOLOGY | 1990年 / 162卷 / 04期

关键词：

COLORECTAL CANCER; INSITU HYBRIDIZATION; MITOCHONDRIA; GENE EXPRESSION; CDNA; INTESTINAL MUCOSA;

D O I：

10.1002/path.1711620406

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

cDNA clones of mRNAs with an abnormal abundance in familial adenomatous polyposis were used to examine the levels and distribution of the mRNAs in tissues from 15 patients with colorectal cancer. Of 12 cloned sequences studied by slot hybridization, one was substantially reduced in tumours compared with normal tissue. Sequence analysis showed this to code for IgA. In situ hybridization was consistent with slot hybridization and immunohistochemistry. Two mitochondrially encoded sequences had distinct distributions detected by in situ hybridization but did not have detectable quantitative differences in whole tumour or mucosa extracts.

引用

页码：309 / 315

页数：7

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