ELECTRON-CAPTURE GAS-CHROMATOGRAPHIC DETERMINATION OF 2-SEC-BUTYL-4,6-DINITROPHENOL (DNBP) RESIDUES IN FEED, TISSUE, AND EXCRETA

Gas chromatographic methods for determining the herbicide DNBP (2-sec-butyl-4,6-dinitrophenol) in feed, blood, urine, feces, and tissues are presented. After extraction the sample was reacted with diazomethane to produce the methyl ether of DNBP prior to analysis by electron-capture gas chromatography. An acid alumina column cleanup was developed to remove interferences from the sample extracts. Average recoveries of greater than 85% were obtained from tissues, excreta, and feed fortified with known amounts of DNBP. Because of apparent binding or salt formation, methodology for freshly fortified and aged feed are also presented. Residue levels of DNBP from rats fed at two feeding levels are also presented. © 1978, American Chemical Society. All rights reserved.