SECONDARY STRUCTURE OF URACIL-DNA GLYCOSYLASE INHIBITOR PROTEIN

被引：12
作者：
BALASUBRAMANIAN, S
BEGER, RD
BENNETT, SE
MOSBAUGH, DW
BOLTON, PH
机构：
[1] WESLEYAN UNIV,DEPT CHEM,MIDDLETOWN,CT 06450
[2] OREGON STATE UNIV,DEPT AGR CHEM,CORVALLIS,OR 97331
[3] OREGON STATE UNIV,DEPT BIOCHEM & BIOPHYS,CORVALLIS,OR 97331
[4] OREGON STATE UNIV,CTR ENVIRONM HLTH SCI,CORVALLIS,OR 97331
来源：
JOURNAL OF BIOLOGICAL CHEMISTRY | 1995年 / 270卷 / 01期
关键词：
D O I：
10.1074/jbc.270.1.296
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The Bacillus subtilis bacteriophage PBS2 uracil-DNA glycosylase inhibitor (Ugi) is an acidic protein of 84 amino acids that inactivates uracil-DNA glycosylase from diverse organisms (Wang, Z., and Mosbaugh, D, W. (1989) J. Biol. Chem. 264, 1163-1171). The secondary structure of Ugi has been determined by solution state multidimensional nuclear magnetic resonance, The protein adopts a single well defined structure consisting of five anti parallel beta-strands and two alpha-helices. Six loop or turn regions were identified that contain approximately one half of the acidic amino acid residues and connect the beta-strands sequentially to one another, The secondary structure suggests which regions of Ugi may be involved in interactions with uracil-DNA glycosylase.
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页码：296 / 303
页数：8
相关论文
共 45 条
[1]  
BENNETT SE, 1993, J BIOL CHEM, V268, P26879
[2]  
BENNETT SE, 1992, J BIOL CHEM, V267, P22512
[3]  
BENNETT SE, 1994, J BIOL CHEM, V269, P21870
[4]  
CARRADONNA SJ, 1981, J BIOL CHEM, V256, P9834
[5]   EXPLORING THE LIMITS OF PRECISION AND ACCURACY OF PROTEIN STRUCTURES DETERMINED BY NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPY [J].
CLORE, GM ;
ROBIEN, MA ;
GRONENBORN, AM .
JOURNAL OF MOLECULAR BIOLOGY, 1993, 231 (01) :82-102
[6]   ENZYMATIC DEGRADATION OF URACIL-CONTAINING DNA .3. PARTIAL-PURIFICATION AND CHARACTERIZATION OF A URACIL DNA N-GLYCOSIDASE FROM BACILLUS-SUBTILIS [J].
CONE, R ;
DUNCAN, J ;
HAMILTON, L ;
FRIEDBERG, EC .
BIOCHEMISTRY, 1977, 16 (14) :3194-3201
[7]   GENERATION OF SINGLE-NUCLEOTIDE REPAIR PATCHES FOLLOWING EXCISION OF URACIL RESIDUES FROM DNA [J].
DIANOV, G ;
PRICE, A ;
LINDAHL, T .
MOLECULAR AND CELLULAR BIOLOGY, 1992, 12 (04) :1605-1612
[8]   TWO-DIMENSIONAL H-1-NMR STUDY OF HUMAN UBIQUITIN - A MAIN CHAIN DIRECTED ASSIGNMENT AND STRUCTURE-ANALYSIS [J].
DISTEFANO, DL ;
WAND, AJ .
BIOCHEMISTRY, 1987, 26 (23) :7272-7281
[9]   MUTAGENIC DEAMINATION OF CYTOSINE RESIDUES IN DNA [J].
DUNCAN, BK ;
MILLER, JH .
NATURE, 1980, 287 (5782) :560-561
[10]   CHEMICAL-SHIFT ASSIGNMENTS AND FOLDING TOPOLOGY OF THE RAS-BINDING DOMAIN OF HUMAN RAF-1 AS DETERMINED BY HETERONUCLEAR 3-DIMENSIONAL NMR-SPECTROSCOPY [J].
EMERSON, SD ;
WAUGH, DS ;
SCHEFFLER, JE ;
TSAO, KL ;
PRINZO, KM ;
FRY, DC .
BIOCHEMISTRY, 1994, 33 (25) :7745-7752
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