SIMULTANEOUS DETERMINATION OF 2 SERINE PROTEINASES BY HYDROPHOBIC INTERACTION CHROMATOGRAPHY

被引：5

作者：

RASPI, G

LOMORO, A

SPINETTI, M

机构：

[1] Dipartimento di Chimica e Chimica Industriale, Università di Pisa, Pisa, 56126

来源：

CHROMATOGRAPHIA | 1993年 / 35卷 / 7-8期

关键词：

COLUMN LIQUID CHROMATOGRAPHY; BOVINE TRYPSIN; PORCINE KALLIKREIN; BOVINE PANCREATIC TRYPSIN INHIBITOR;

D O I：

10.1007/BF02278589

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The possibility of determining more than one enzyme at the same time has been examined. A new approach, based on the measurement of a direct and specific chromatographic signal obtained by hydrophobic interaction chromatography (HIC) of the stable complex formed with the inhibitor aprotinin, is proposed. A basic procedure for the quantitative determination of trypsin and kallikrein, taken as models, is described. The method is precise with a mean coefficient of variation of 3.1 % and 3.5 % for trypsin and kallikrein, respectively; the limit of determination for both enzymes is 0.17 nmol ml-1 in the original sample.

引用

页码：381 / 386

页数：6

共 7 条

[1]

Birk Y, 1976, Methods Enzymol, V45, P700

[2] P-NITROPHENYL-P'-GUANIDINOBENZOATE HCL - A NEW ACTIVE SITE TITRANT FOR TRYPSIN [J].