XKLP1, A CHROMOSOMAL XENOPUS KINESIN-LIKE PROTEIN ESSENTIAL FOR SPINDLE ORGANIZATION AND CHROMOSOME POSITIONING

被引：207

作者：

VERNOS, I

RAATS, J

HIRANO, T

HEASMAN, J

KARSENTI, E

WYLIE, C

机构：

[1] EUROPEAN MOLEC BIOL LAB,CELL BIOPHYS PROGRAMME,D-69117 HEIDELBERG,GERMANY

[2] UNIV MINNESOTA,SCH MED,DEPT CELL BIOL & NEUROANAT,MINNEAPOLIS,MN 55455

[3] UNIV MINNESOTA,SCH MED,DEPT PEDIAT,MINNEAPOLIS,MN 55455

[4] UNIV CALIF SAN FRANCISCO,DEPT PHARMACOL,SAN FRANCISCO,CA 94143

[5] WELLCOME CANC RES CAMPAIGN INST,CAMBRIDGE CB2 1QR,ENGLAND

来源：

CELL | 1995年 / 81卷 / 01期

基金：

英国惠康基金;

关键词：

D O I：

10.1016/0092-8674(95)90376-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Xklp1 is a novel Xenopus kinesin-like protein with a motor domain at the amino terminus, nuclear localisation sequences in the stalk, and a putative zinc fingerlike sequence in the tail. It is nuclear during interphase and chromosomal during mitosis. During late anaphase, a fraction of the protein relocalizes to the spindle interzone and accumulates in the midbody during telophase. Depletion of Xklp1 protein by antisense oligo knockout in oocytes leads to defective mitosis during the first cell cycles following fertilization. The bipolarity of spindles assembled in vitro in the presence of anti-Xklp1 antibodies is unstable, and the chromosomes fail to congress on the metaphase plate.

引用

页码：117 / 127

页数：11

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