HALOINCOLA-SACCHAROLYTICA SUBSP SENEGALENSIS SUBSP NOV, ISOLATED FROM THE SEDIMENTS OF A HYPERSALINE LAKE, AND EMENDED DESCRIPTION OF HALOINCOLA-SACCHAROLYTICA

A new halophilic chemoorganotrophic bacterium (strain H150(T) [T = type strain]) related to Haloincola saccharolytica was isolated from the hypersaline sediments of Retba Lake in Senegal. This organism was a rod-shaped, motile, non-spore-forming, gram-negative obligate anaerobe that grew optimally in the presence of 10% NaCl and at 40 degrees C. The DNA base composition was 31.7 +/- 0.3 mol% guanine plus cytosine. The fermentation products from glucose were acetate, H-2, and CO2. The fermentable substrates included cellobiose, fructose, glucose, maltose, lactose, glycerol, mannitol, mannose, ribose, raffinose, and sucrose. Penicillin G, cephalosporin C, novobiocin, vancomycin, and chloramphenicol inhibited growth. As determined by DNA-DNA hybridization, strain H150(T) was 71% related to H. saccharolytica, with Delta T-m value of 6.0 degrees C. However, strain H150(T) exhibited marked phenotypic differences, particularly in the range of substrates used, when it was compared with the type species of the genus Haloincola. For this isolate we propose the name Haloincola saccharolytica subsp. senegalensis subsp. nov.; strain H150 (= DSM 7379) is the type strain of this taxon.