APPLICATION OF CAPILLARY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY TO BIOTECHNOLOGY, WITH REFERENCE TO THE ANALYSIS OF RECOMBINANT DNA-DERIVED HUMAN GROWTH-HORMONE

被引：10
作者：
BATTERSBY, JE
GUZZETTA, AW
HANCOCK, WS
机构：
[1] Genentech Inc., South San Francisco, CA 94080
来源：
JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 662卷 / 02期
关键词：
D O I：
10.1016/0378-4347(94)00312-2
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Using capillary HPLC, femtomole amounts of recombinant DNA-derived human growth hormone (rhGH) have been successfully detected from solutions at nanomolar concentrations. The separation used capillaries of 15 cm x 320 mu m I.D. and detection was with a UV absorbance detector containing a capillary Z-shaped flow-cell. A sample of rhGH that was recovered from rat serum was analyzed by capillary reversed-phase HPLC, using both acidic- and neutral-pH mobile phases, as well as by capillary ion-exchange chromatography. When compared to HPLC separations performed at flow-rates of 1 ml/min, the sensitivity of the detection was increased 200 times, without any loss in resolution. Sub-microgram amounts of rhGH were also analyzed by tryptic mapping using capillary HPLC and peptides were identified by capillary LC-MS.
引用
收藏
页码：335 / 342
页数：8
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