THE SIMULTEST APPROACH FOR TESTING MUTAGENS IN THE SALMONELLA MICROTITRE FLUCTUATION ASSAY

被引：6

作者：

MCPHERSON, MF ^{[1
]}

NESTMANN, ER ^{[1
]}

机构：

[1] DEPT NATL HLTH & WELFARE CANADA ENVIRONM HLTH DIRECTORATE,MUTAGENESIS SECT,OTTAWA,ONTARIO,CANADA

来源：

ENVIRONMENTAL AND MOLECULAR MUTAGENESIS | 1990年 / 16卷 / 01期

关键词：

combinations of strains; fluctuation vs. plate incorporation tests; quantity index; reduced workload; sensitivity index; testing simultaneously;

D O I：

10.1002/em.2850160104

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The concept of combining several histidine‐dependent Salmonella strains in a single test, the SIMULTEST, has been applied to the microtitre fluctuation test. The activity of five mutagens was determined in strains TA97, TA98, TA100, and TA102 individually as well as in a SIMULTEST mixture. All five compounds were mutagenic in the SIMULTEST, demonstrating the utility of this time and labour‐saving approach of combining strains for testing with this method. The microtitre fluctuation SIMULTEST results were quantitatively comparable to those of the SIMULTEST Salmonella/microsome plate test. The microtitre fluctuation test compared with the plate incorporation assay generally showed more favourable “sensitivity” and “quantity” indices in that four of the five chemicals tested in the fluctuation test were mutagenic at lower doses than in the plate test. Copyright © 1990 Wiley‐Liss, Inc., A Wiley Company

引用

页码：21 / 25

页数：5

共 16 条

[1] THE DIFFERENTIAL MUTAGENICITY OF ISONIAZID IN FLUCTUATION ASSAYS AND SALMONELLA PLATE TESTS [J].