CLONING, DISRUPTION AND SEQUENCE OF THE GENE ENCODING YEAST C-5 STEROL DESATURASE

被引：129
作者：
ARTHINGTON, BA
BENNETT, LG
SKATRUD, PL
GUYNN, CJ
BARBUCH, RJ
ULBRIGHT, CE
BARD, M
机构：
[1] INDIANA UNIV PURDUE UNIV,DEPT BIOL,1125 E 38 ST,INDIANAPOLIS,IN 46205
[2] VET AFFAIRS MED CTR,INDIANAPOLIS,IN 46202
[3] ELI LILLY & CO,LILLY RES LAB,INDIANAPOLIS,IN 46285
[4] MERRELL DOW PHARMACEUT INC,RES INST,CINCINNATI,OH 45215
来源：
GENE | 1991年 / 102卷 / 01期
关键词：
ERGOSTEROL BIOSYNTHESIS; SPARKING FUNCTION; RECOMBINANT DNA;
D O I：
10.1016/0378-1119(91)90535-J
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The ERG3 gene from Saccharomyces cerevisiae has been cloned by complementation of an erg3-2 mutation. ERG3 is the putative gene encoding the C-5 sterol desaturase required for ergosterol biosynthesis. The functional gene has been localized on a 2.5-kb HindIII-BamHI fragment containing an open reading frame comprising 365 amino acids. Gene disruption resulting from a deletion/substitution demonstrates that ERG3 is not essential for cell viability or the sparking function.
引用
收藏
页码：39 / 44
页数：6
相关论文
共 20 条
[1]  
ASHMAN WH, 1991, IN PRESS LIPIDS
[2]   INHIBITION OF ERGOSTEROL BIOSYNTHESIS IN SACCHAROMYCES-CEREVISIAE AND USTILAGO-MAYDIS BY TRIDEMORPH, FENPROPIMORPH AND FENPROPIDIN [J].
BALOCH, RI ;
MERCER, EI ;
WIGGINS, TE ;
BALDWIN, BC .
PHYTOCHEMISTRY, 1984, 23 (10) :2219-2226
[3]  
BARANY F, 1988, DNA PROTEIN ENG TECH, V1, P29
[4]  
BARD M, 1972, Journal of Bacteriology, V111, P649
[5]   BIOSYNTHESIS OF TERPENES AND STEROIDS .9. STEROLS OF SOME MUTANT YEASTS AND THEIR RELATIONSHIP TO BIOSYNTHESIS OF ERGOSTEROL [J].
BARTON, DHR ;
CORRIE, JET ;
WIDDOWSON, DA ;
BARD, M ;
WOODS, RA .
JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 1974, (11) :1326-1333
[6]   STERILE HOST YEASTS (SHY) - EUKARYOTIC SYSTEM OF BIOLOGICAL CONTAINMENT FOR RECOMBINANT DNA EXPERIMENTS [J].
BOTSTEIN, D ;
FALCO, SC ;
STEWART, SE ;
BRENNAN, M ;
SCHERER, S ;
STINCHCOMB, DT ;
STRUHL, K ;
DAVIS, RW .
GENE, 1979, 8 (01) :17-24
[7]  
CARMAN GM, 1989, ANNU REV BIOCHEM, V58, P635, DOI 10.1146/annurev.biochem.58.1.635
[8]   ISOLATION OF MUTANT SACCHAROMYCES-CEREVISIAE STRAINS THAT SURVIVE WITHOUT SPHINGOLIPIDS [J].
DICKSON, RC ;
WELLS, GB ;
SCHMIDT, A ;
LESTER, RL .
MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (05) :2176-2181
[9]   THE YEAST GENE ERG6 IS REQUIRED FOR NORMAL MEMBRANE-FUNCTION BUT IS NOT ESSENTIAL FOR BIOSYNTHESIS OF THE CELL-CYCLE-SPARKING STEROL [J].
GABER, RF ;
COPPLE, DM ;
KENNEDY, BK ;
VIDAL, M ;
BARD, M .
MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (08) :3447-3456
[10]   TRANSFORMATION OF INTACT YEAST-CELLS TREATED WITH ALKALI CATIONS [J].
ITO, H ;
FUKUDA, Y ;
MURATA, K ;
KIMURA, A .
JOURNAL OF BACTERIOLOGY, 1983, 153 (01) :163-168
12