STUDIES ON NONCOVALENT ASSOCIATIONS OF IMMUNOSUPPRESSIVE DRUGS WITH SERUM-ALBUMIN USING PNEUMATICALLY ASSISTED ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY

被引：18
作者：
BAKHTIAR, R
STEARNS, RA
机构：
[1] Department of Drug Metabolism (I), Merck Research Laboratories, Rahway, New Jersey, 07065-0900
来源：
RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1995年 / 9卷 / 03期
关键词：
D O I：
10.1002/rcm.1290090313
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Pneumatically assisted electrospray ionization (ion-spray) mass spectrometry was employed in the detection of non-covalent interactions of inmunosuppressive drugs with proteins. Non-covalent complexes of albumin (rat and bovine) with FK506, dihydro-FK506, and FK520 were observed. No complexation was detected between albumin and cyclosporin A (CsA), even at a protein-ligand molar ratio of 1:20. It is suggested that the lack of detection of an albumin-CsA non covalent complex may be attributed to dissociation of the weakly associated species during ion vaporization. Nonetheless, preliminary results indicate that ion-spray mass spectrometry has potential in qualitative evaluation of drug-plasma protein interaction.
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收藏
页码：240 / 244
页数：5
相关论文
共 58 条
  • [1] DOES THE OBSERVATION OF NONCOVALENT COMPLEXES BETWEEN BIOMOLECULES BY ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY NECESSARILY REFLECT SPECIFIC SOLUTION INTERACTIONS[J]. APLIN, RT;ROBINSON, CV;SCHOFIELD, CJ;WESTWOOD, NJ. JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1994(20)
  • [2] DIRECT OBSERVATION OF A TERNARY COMPLEX BETWEEN THE DIMERIC ENZYME HIV-1 PROTEASE AND A SUBSTRATE-BASED INHIBITOR[J]. BACA, M;KENT, SBH. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992(10)
  • [3] APPLICATION OF THERMALLY ASSISTED ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY FOR DETECTION OF NONCOVALENT COMPLEXES OF BOVINE SERUM-ALBUMIN WITH GROWTH-HORMONE RELEASING-FACTOR AND OTHER BIOLOGICALLY-ACTIVE PEPTIDES[J]. BACZYNSKYJ, L;BRONSON, GE;KUBIAK, TM. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1994(03)
  • [4] ANALYSIS OF DOUBLE-STRANDED OLIGONUCLEOTIDES BY ELECTROSPRAY MASS-SPECTROMETRY[J]. BAYER, E;BAUER, T;SCHMEER, K;BLEICHER, K;MALER, M;GAUS, HJ. ANALYTICAL CHEMISTRY, 1994(22)
  • [5] CYCLOSPORINE-A AND FK506 - MOLECULAR MECHANISMS OF IMMUNOSUPPRESSION AND PROBES FOR TRANSPLANTATION BIOLOGY[J]. BIERER, BE;HOLLANDER, G;FRUMAN, D;BURAKOFF, SJ. CURRENT OPINION IN IMMUNOLOGY, 1993(05)
  • [6] BOREL JF, 1989, PHARMACOL REV, V41, P239
  • [7] ION SPRAY INTERFACE FOR COMBINED LIQUID CHROMATOGRAPHY/ATMOSPHERIC PRESSURE IONIZATION MASS-SPECTROMETRY[J]. BRUINS, AP;COVEY, TR;HENION, JD. ANALYTICAL CHEMISTRY, 1987(22)
  • [8] CYCLOSPORINE - A LI-SPECIFIC AND CA-SPECIFIC IONOPHORE[J]. BURGER, HM;SEEBACH, D. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH, 1994(04)
  • [9] OBSERVATION OF LARGE MULTIMERS IN THE ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY OF PEPTIDES[J]. BUSMAN, M;KNAPP, DR;SCHEY, KL. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1994(02)
  • [10] INVESTIGATING THE NONCOVALENT INTERACTION OF CYTIDYLIC ACIDS, WITH RIBONUCLEASE-A BY ELECTROSPRAY MASS-SPECTROMETRY[J]. CAMILLERI, P;HASKINS, NJ. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1993(07)
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