DETECTION OF RIBOSOMAL DNA SITES IN LENTIL AND CHICKPEA BY FLUORESCENT IN-SITU HYBRIDIZATION

被引：31

作者：

ABBO, S ^{[1
]}

MILLER, TE ^{[1
]}

READER, SM ^{[1
]}

DUNFORD, RP ^{[1
]}

KING, IP ^{[1
]}

机构：

[1] JOHN INNES CTR PLANT SCI RES, NORWICH NR4 7UJ, ENGLAND

来源：

GENOME | 1994年 / 37卷 / 04期

关键词：

CHICKPEA; LENTIL; RDNA SITES; NUCLEOLUS ORGANIZER REGION; FLUORESCENT IN SITU HYBRIDIZATION; PRIMER-MEDIATED IN SITU HYBRIDIZATION;

D O I：

10.1139/g94-101

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Hybridization sites of an rDNA probe coding for the 18S, 5.8S, and 26S genes were detected on lentil and chickpea somatic chromosomes using fluorescent in situ hybridization. One pair of hybridization sites was detected in cultivated lentil Lens culinaris L. and wild lentil L. orientalis (Boiss.) Hand.-Mazz., and in both the hybridization sites of the ribosomal probe correspond to the secondary constriction. In cultivated chickpea Cicer arietinum three pairs of rDNA sites were detected and in the wild C. reticulatum two pairs were detected. The karyotypic relationship between the cultivated C. arietinum and its wild progenitor C. reticulatum is discussed.

引用

页码：713 / 716

页数：4

共 28 条

[1] PRIMER-INDUCED IN-SITU HYBRIDIZATION TO PLANT CHROMOSOMES [J].