CONTRIBUTION OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY TO THE IDENTIFICATION OF SOME CORYNEBACTERIUM SPECIES BY COMPARISON OF THEIR CORYNOMYCOLIC ACID PATTERNS

被引：5

作者：

DEBRIEL, D ^{[1
]}

COUDERC, F ^{[1
]}

RIEGEL, P ^{[1
]}

GALLION, C ^{[1
]}

LANGS, JC ^{[1
]}

JEHL, F ^{[1
]}

机构：

[1] CTR RECH BIOCHIM & GENET CELLULAIRES,F-31062 TOULOUSE,FRANCE

来源：

RESEARCH IN MICROBIOLOGY | 1992年 / 143卷 / 02期

关键词：

CORYNOMYCOLIC ACID; CORYNEBACTERIUM; HPLC; MASS SPECTROMETRY; IDENTIFICATION;

D O I：

10.1016/0923-2508(92)90008-C

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Reverse-phase high-performance liquid chromatography of corynomycolic acids provided a specific pattern for each Corynebacterium species studied. These data suggest that a fast and reproducible procedure is now available for bacteriological identification at the genus and at the species level of corynomycolic-acid-containing bacteria. Mass spectrometry analysis of post-column collected fractions provided the order of elution of some corynomycolic acids and isomers and showed the high specificity of the chromatographic assay which could be used for the routine bacteriological identification of some species belonging to the genus Corynebacterium.

引用

页码：191 / 198

页数：8

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