A SIMPLE AND INEXPENSIVE METHOD FOR ASSESSING INVITRO CANDIDACIDAL ACTIVITY OF LEUKOCYTES

被引：9

作者：

CUTLER, JE ^{[1
]}

THOMPSON, BD ^{[1
]}

机构：

[1] MONTANA STATE UNIV, DEPT MICROBIOL, BOZEMAN, MT 59717 USA

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1984年 / 66卷 / 01期

关键词：

D O I：

10.1016/0022-1759(84)90244-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Candidacidal activity of mouse neutrophils and macrophages was determined directly in microtiter plates. After a suitable period of interaction between phagocytic cells and Candida albicans in the wells, the mouse cells were lysed with distilled water and corn meal agar was added to each well. Following incubation at 37.degree. C, viability was assessed using an inverted microscope and counting the number of germ tubes or microcolonies which developed. This method does not use radioisotopes or vital stains, and should be applicable to other genera of yeasts.

引用

页码：27 / 33

页数：7

共 14 条

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