EXTRACTION AND SECONDARY METABOLITE ANALYSIS OF COSCINIUM FENESTRATUM (GAERTN.) COLEBR: AN IMPORTANT MEDICINAL PLANT OF WESTERN GHATS

被引：7

作者：

Goveas, Santhosh Wilson ^{[1
]}

Abraham, Asha ^{[1
]}

机构：

[1] St Aloysius Coll, Post Grad Dept Biotechnol, Father George Albuquerque Pai Cell & Mol Biol Lab, Mangalore 575003, Karnataka, India

来源：

INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES AND RESEARCH | 2014年 / 5卷 / 08期

关键词：

Coscinium fenestratum; Berberine; Phytochemicals; Secondary metabolites; HPLC; Phenolics; medicinal plant;

D O I：

10.13040/IJPSR.0975-8232.5(8).3484-89

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The aim of this study was to extract the secondary metabolites of Coscinium fenestratum stem extracts and to determine the alkaloid berberine using HPLC. Phytochemical analysis was carried for acetone, chloroform, methanol and Aqueous fractions of stem extracts. Total phenolic content and total flavonoid contents were estimated using standard Gallic acid and Quercitin respectively. TLC and HPLC were performed for alkaloid to determine the berberine content present in the stem extract. Preliminary phytochemical screening revealed the presence of alkaloids, saponins, steroids, phenolic substances and cardiac glycosides. Steroids and tannins were absent in the stem extract. The stem methanolic extract of C. fenestratum contained large amount of phenolics 18.35 +/- 0.56mg GAE/g extract and the flavonoid was found to be 12.8 +/- 0.88 mg QE/g extract respectively. The concentration of berberine in the stem was found to be 0.2462%.

引用

页码：3484 / 3489

页数：6

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