PRODUCTION AND TUMOR-BINDING CHARACTERIZATION OF A CHIMERIC ANTI-CEA FAB EXPRESSED IN ESCHERICHIA-COLI

被引：27

作者：

CHESTER, KA ^{[1
]}

ROBSON, L ^{[1
]}

KEEP, PA ^{[1
]}

PEDLEY, RB ^{[1
]}

BODEN, JA ^{[1
]}

BOXER, GM ^{[1
]}

HAWKINS, RE ^{[1
]}

BEGENT, RHJ ^{[1
]}

机构：

[1] MRC CTR,CAMBRIDGE CB2 2QH,CAMBS,ENGLAND

来源：

INTERNATIONAL JOURNAL OF CANCER | 1994年 / 57卷 / 01期

关键词：

D O I：

10.1002/ijc.2910570113

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A recombinant chimeric Fab (rcFab), with Fv derived from the monoclonal A5B7 antibody to carcinoembryonic antigen (CEA), and with human CHI and C kappa was doned into pUC 19 and expressed in Escherichia coli. rcFab (10 to 12 mg per litre) was produced in bacterial culture fluid, and functional purified rcFab was isolated by affinity chromatography (using antibody to human C kappa) and size-exclusion gel filtration. The rcFab did not show reduced affinity for CEA, and reacted with human colorectal tumours showing a typical anti-CEA pattern by immunocytochemistry; it was also stable after iodination. Biodistribution studies in nude mice bearing human tumour xenografts showed no toxicity and good tumour localization. Therapeutic ratios at early time points were better than those obtained with whole murine antibody. The results demonstrate that bacterially produced anti-CEA Fab is of use for tumour targeting.

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页码：67 / 72

页数：6

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