HUMAN SEMINAL PLASMA ZN-ALPHA-2-GLYCOPROTEIN - ITS PURIFICATION AND PROPERTIES AS COMPARED WITH HUMAN PLASMA ZN-ALPHA-2-GLYCOPROTEIN

被引：40

作者：

OHKUBO, I

NIWA, M

TAKASHIMA, A

NISHIKIMI, N

GASA, S

SASAKI, M

机构：

[1] NAGOYA CITY UNIV, SCH MED, DEPT DERMATOL, NAGOYA, AICHI 467, JAPAN

[2] NAGOYA CITY UNIV, SCH MED, DEPT OBSTET & GYNECOL, NAGOYA, AICHI 467, JAPAN

[3] HOKKAIDO UNIV, SCH MED, INST CANC, BIOCHEM LAB, SAPPORO, HOKKAIDO 060, JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1034卷 / 02期

关键词：

(Human); Amino acid sequence; Carbohydrate content; Glycoprotein; Seminal plasma; Zinc-α[!sub]2[!/sub]-glycoprotein;

D O I：

10.1016/0304-4165(90)90069-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

On the basis of the datum that the level of Zn-β2-glycoprotein (Znα2gp) in human seminal plasma was about 6-times higher than that in adult serum, Znα2gp was purified from fresh human seminal plasma approx. 70-fold with 60% yield over seminal plasma by DEAE-Sephacel, Zn-chelate Sepharose 4B and DEAE-5PW column chromatographies. The molecular weight of seminal plasma Znα2gp was 50 000 on Superose column chromatography, and 40 500 and 41 500 on SDS-polyacrylamide gel electrophoresis in the absence and presence of β-mercaptoethanol, respectively. Plasma Znα2gp is a glycoprotein, while the protein from seminal plasma does not contain carbohydrate. The amino acid sequence of the first 17 residues of seminal plasma Znα2gp was Glu-Asn-Gln-Asp-Gly-Asn-Tyr-Ser-Leu-Thr-Tyr-Ile-Tyr-Thr-Gly-Leu-Ser. This sequence was completely identical with the amino acid residues from Glu-2 to Ser-18 in the N-terminal amino acid sequence of plasma Znα2gp. These data suggest that bot Znα2gps in plasma and seminal plasma may be expressed from one gene, but their posttranslational modifications are different. © 1990.

引用

页码：152 / 156

页数：5

共 17 条

[1] COMPLETE AMINO-ACID SEQUENCE OF HUMAN-PLASMA ZN-ALPHA-2-GLYCOPROTEIN AND ITS HOMOLOGY TO HISTOCOMPATIBILITY ANTIGENS [J].