SOLID-PHASE EXTRACTION OF ARGININE VASOTOCIN AND ISOTOCIN IN FISH SAMPLES AND SUBSEQUENT GRADIENT REVERSED-PHASE HIGHPERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION

被引：19
作者：
KULCZYKOWSKA, E
机构：
[1] Marine Biology Center, Polish Academy of Sciences, 81-347 Gdynia
来源：
JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 673卷 / 02期
关键词：
D O I：
10.1016/0378-4347(95)00276-3
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Gradient high-performance liquid chromatography (HPLC) preceded by a solid-phase extraction (SPE) step is described for determining arginine vasotocin and isotocin, the neurohypophysial nonapeptides, in fish plasma samples. The combination of these two methods significantly improves the separation and increases the sensitivity of the assay. The proposed assay may be a useful alternative for analysis of similar nonapeptides in plasma without the use of radioisotopes, while taking into consideration a difference in detection sensitivity.
引用
收藏
页码：289 / 293
页数：5
相关论文
共 38 条
[1]  
BABIKER MM, 1980, GEN COMP ENDOCR, V40, P337
[2]  
Bentley P.J., 1971, ZOOPHYSIOLOGY ECOLOG, V1, P199
[3]   VASOTOCIN AND HYDRIN-2 (VASOTOCINYL-GLY) IN THE AFRICAN TOAD BUFO-REGULARIS - STUDY UNDER VARIOUS ENVIRONMENTAL-CONDITIONS [J].
CHAUVET, J ;
OUEDRAOGO, Y ;
MICHEL, G ;
ACHER, R .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-PHYSIOLOGY, 1993, 104 (03) :497-502
[4]   THE CARDIOVASCULAR AND RENAL EFFECTS OF HYDRINS AND ARGININE VASOTOCIN IN FROGS [J].
CHIU, KW ;
LEE, YC ;
PANG, PKT .
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 1993, 91 (01) :1-7
[5]   ISOCRATIC REVERSE-PHASE HPLC SEPARATION AND RIA USED IN THE ANALYSIS OF NEUROPEPTIDES IN BRAIN-TISSUE [J].
DECEBALLOS, ML ;
TAYLOR, MD ;
JENNER, P .
NEUROPEPTIDES, 1991, 20 (03) :201-209
[6]   PLASMA ARGININE VASOTOCIN, PROGESTERONE, AND LUTEAL DEVELOPMENT DURING PREGNANCY IN THE VIVIPAROUS LIZARD TILIQUA-RUGOSA [J].
FERGUSSON, B ;
BRADSHAW, SD .
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 1991, 82 (01) :140-151
[7]   NEUROHYPOPHYSEAL HORMONAL-CONTROL OF CORTISOL SECRETION IN THE TELEOST CARASSIUS-AURATUS [J].
FRYER, JN ;
LEUNG, E .
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 1982, 48 (04) :425-431
[8]  
GOYD SEK, 1994, NEUROENDOCRINOLOGY, V60, P150
[9]  
GREY DA, 1994, GEN COMP ENDOCR, V93, P122
[10]   GI PROTEIN MEDIATES ADENYLATE-CYCLASE INHIBITION BY NEUROHYPOPHYSEAL HORMONES IN FISH GILL [J].
GUIBBOLINI, ME ;
LAHLOU, B .
PEPTIDES, 1992, 13 (05) :865-871
1234