AEROSOL MATRIX-ASSISTED LASER-DESORPTION IONIZATION - EFFECTS OF ANALYTE CONCENTRATION AND MATRIX-TO-ANALYTE RATIO

被引：21

作者：

BEESON, MD ^{[1
]}

MURRAY, KK ^{[1
]}

RUSSELL, DH ^{[1
]}

机构：

[1] TEXAS A&M UNIV,DEPT CHEM,COLLEGE STN,TX 77843

来源：

ANALYTICAL CHEMISTRY | 1995年 / 67卷 / 13期

关键词：

D O I：

10.1021/ac00109a012

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We have recently developed an aerosol-liquid introduction interface for matrix-assisted laser desorption ionization (MALDI) mass spectrometry. In this study, we examine the effect of matrix-to-analyte ratio and analyte concentration on analyte ion yield. These studies were performed using bradykinin, gramicidin S, bovine insulin, and myoglobin as analytes and alpha-cyano-4-hydroxycinnamic acid and 4-nitroaniline as matrices. The optimum matrix-to-analyte molar ratio for aerosol MALDI was determined to be 10-100:1, which is lower than that typically used for conventional surface MALDI (100-10 000:1). The ion yield was found to be a nonlinear function of analyte concentration. Possible explanations for these observations are discussed.

引用

页码：1981 / 1986

页数：6

共 43 条

[1] CONDENSATION NUCLEATION LIGHT-SCATTERING - A NEW APPROACH TO DEVELOPMENT OF HIGH-SENSITIVITY, UNIVERSAL DETECTORS FOR SEPARATIONS [J].