PARAMETERS INVOLVED IN THE INVITRO IMMUNIZATION OF TONSILAR LYMPHOCTYES - EFFECTS OF RIL-2 AND MURAMYL DIPEPTIDE

被引：3

作者：

CARROLL, K ^{[1
]}

PROSSER, E ^{[1
]}

OKENNEDY, R ^{[1
]}

机构：

[1] DUBLIN CITY UNIV,SCH BIOL SCI,DUBLIN 9,IRELAND

来源：

HYBRIDOMA | 1990年 / 9卷 / 01期

关键词：

D O I：

10.1089/hyb.1990.9.81

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the in vitro immunization of human tonsilar lymphocytes is described. This method allows for the sensitization of B-cells against various antigens under serum-free medium conditions. The medium is supplemented with defined concentrations of antigen, rIL-2 and muramyl dipeptide to boost the immune responses. This method may prove useful for the generation of antigen reactive B-cells for use in human monoclonal antibody production and the study of the effects of other lymphokines on the in vitro immune response.

引用

页码：81 / 89

页数：9

共 12 条

[1]

BAHR GM, 1986, FASEB J, V45, P2541

[2] HUMAN MONOCLONAL-ANTIBODIES PRODUCED BY PRIMARY INVITRO IMMUNIZATION OF PERIPHERAL-BLOOD LYMPHOCYTES [J].