COMPETITIVE IMMUNOLOGICAL DETECTION OF PROGESTERONE BY MEANS OF THE ION-STEP INDUCED RESPONSE OF AN IMMUNOFET

被引：12

作者：

SCHASFOORT, RBM ^{[1
]}

KELDERMANS, CEJM ^{[1
]}

KOOYMAN, RPH ^{[1
]}

BERGVELD, P ^{[1
]}

GREVE, J ^{[1
]}

机构：

[1] UNIV TWENTE,DEPT ELECT ENGN,7500 AE ENSCHEDE,NETHERLANDS

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 1990年 / 1卷 / 1-6期

关键词：

D O I：

10.1016/0925-4005(90)80232-O

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The ion-step method was originally designed to measure immunoreactions via the change in charge density which occurs in an antibody loaded membrane, deposited on an ISFET, upon reaction with a charged antigen. In this paper we show the possibility of detecting an immunoreaction involving a non-charged antigen, progesterone, by using competitive binding of progesterone and a charged progesterone-lysozyme conjugate in the membrane. Both the immunoreaction and the non-specific binding could unequivocally be measured. The detection limit was approximately 10-8 M of progesterone in the sample solution. This proves that the ion-step method is suitable for detection of both charged and non-charged antigens. © 1990.

引用

页码：368 / 372

页数：5