IMMUNOPEROXIDASE PROCEDURES FOR RAPID DETECTION OF BOVINE VIRAL DIARRHEA-MUCOSAL DISEASE VIRUS-ANTIGEN

被引：3

作者：

SAINO, H

WATANABE, H

IKEHATA, T

机构：

[1] Abashiri Livestock Hygiene Service Center, 323-5 Taisyo, Kitami

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 1994年 / 56卷 / 04期

关键词：

BOVINE VIRAL DIARRHEA; IMMUNOPEROXIDASE; PERSISTENT INFECTION;

D O I：

10.1292/jvms.56.805

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Bovine viral diarrhea-mucosal disease (BVD-MD) virus antigen was detected in bovine fetal muscular cells from sera of 42 cattle persistently infected with noncytopathic BVD-MD virus by the indirect immunoperoxidase procedure (IIP) but not from sera of 100 apparently healthy cattle. These findings agreed with the results obtained by the interference method. Furthermore, BVD-MD virus antigens were detectable in the smears of buffy coats by the IIP procedure and in the formalin-fixed tissue sections by the avidin-biotin-peroxidase complex procedure. These procedures are therefore expected to be useful for rapid detection of BVD-MD virus antigen.

引用

页码：805 / 807

页数：3

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