ESTIMATION OF SPERM CONCENTRATION OF RAINBOW-TROUT, WHITEFISH AND YELLOW PERCH USING A SPECTROPHOTOMETRIC TECHNIQUE

被引：188
作者：
CIERESZKO, A
DABROWSKI, K
机构：
[1] OHIO STATE UNIV,SCH NAT RESOURCES,2021 COFFEY RD,COLUMBUS,OH 43210
[2] POLISH ACAD SCI,CTR AGROTECHNOL & VET SCI,DEPT REPROD ENDOCRINOL,OLSZTYN,POLAND
来源：
AQUACULTURE | 1993年 / 109卷 / 3-4期
关键词：
D O I：
10.1016/0044-8486(93)90175-X
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
Milt was collected individually from rainbow trout (Oncorhynchus mykiss), whitefish (Coregonus clupeaformis) and yellow perch (Perca flavescens) and sperm density measured by three different methods: measuring spermatocrit value, counting sperm using a Neubauer counting chamber, and by measuring absorbance at 505 nm. Sperm concentration significantly differed between yellow perch (41.58 +/- 3.5 x 10(9) ml-1) and the two other species, rainbow trout (11.80 +/- 6.19 X 10(9)) and whitefish (7.95 +/- 2.5 7 x 10(9)). In diluted yellow perch sperm and directly sampled sperm of whitefish and rainbow trout, correlation coefficients were highly significant between spermatocrit, counting, and the spectrophotometric method of measurement. It is suggested that sperm concentration measured by optical density offers a quick and accurate method of determining sperm concentration.
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页码：367 / 373
页数：7
相关论文
共 16 条
  • [1] BILLARD R, 1971, ANN BIOL ANIM BIOCH, V11, P190
  • [2] BOUCK GR, 1976, T AM FISH SOC, V105, P534, DOI 10.1577/1548-8659(1976)105<534:EOSSCB>2.0.CO
  • [3] 2
  • [4] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
  • [5] Foote R.H., 1974, REPROD FARM ANIMALS, P409
  • [6] FOOTE RH, 1964, AM J VET RES, V25, P558
  • [7] HAAG FRIEDRICH M., 1959, JOUR AMER VET MED ASSOC, V134, P314
  • [8] Koenig S.D., 1978, AM FISH SOC SPEC PUB, V11, P177
  • [9] PROTEINS OF SEMINAL FLUID AND SPERMATOZOA IN THE TROUT (ONCORHYNCHUS-MYKISS) - PARTIAL CHARACTERIZATION AND VARIATIONS
    LOIR, M
    LABBE, C
    MAISSE, G
    PINSON, A
    BOULARD, G
    MOUROT, B
    CHAMBEYRON, F
    [J]. FISH PHYSIOLOGY AND BIOCHEMISTRY, 1990, 8 (06) : 485 - 495
  • [10] SEASONAL-CHANGES IN THE QUALITY OF RAINBOW-TROUT (SALMO-GAIRDNERI) SEMEN - EFFECT OF A DELAY IN STRIPPING ON SPERMATOCRIT, MOTILITY, VOLUME AND SEMINAL PLASMA CONSTITUENTS
    MUNKITTRICK, KR
    MOCCIA, RD
    [J]. AQUACULTURE, 1987, 64 (02) : 147 - 156
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