PROTEIN COMPLEX AND ESTERASE ISOENZYME PATTERNS OF ALLIUM-SATIVUM L CULTIVARS AND CLONES-REGENERANTS

Protein complex patterns of cloves and esterase isoenzyme patterns of apical buds of cloves were studied with Czechoslovak virus-free cultivars of Allium sativum L. and the wild species A. longicuspis Regel, Similarly, four clones-regenerants obtained using explant culture techniques from A. sativum L. cv. Bzenecký paličák (two somaclones and two clones derived from plants regenerated from meristem cultures treated in vitro with colchicine) differing in their ploidy, morphology, and yields were studied. Immunophoreograms of protein complexes of the A. sativum L. cultivars under investigation differed from one another in the number and mobility of protein fractions in both the cathodic and anodic regions and thus these cultivars can be distinguished. On the basis of esterase isoenzyme patterns, the Czechoslovak cultivars of A. sativum L. can be arranged into three groups - bolting winter cultivars with broad leaves, non-bolting winter cultivars with broad leaves, and non-bolting spring cultivars with narrow leaves. All the clones-regenerants showed the same protein complex and esterase isoenzyme patterns as their original cultivar. A. longicuspis Regel markedly differed in its protein complex and esterase isoenzyme patterns from all the other genotypes studied. Received May 17, 1989: accepted January 5, 1990 © 1990 Kluwer Academic Publishers.