A sandwich enzyme-linked immunosorbent assay for human seminal gamma-glutamyl transpeptidase

被引：3

作者：

Abe, S ^{[1
]}

Gunji, H ^{[1
]}

Fujita, T ^{[1
]}

Hiraiwa, K ^{[1
]}

机构：

[1] FUKUSHIMA MED COLL,DEPT BIOCHEM,FUKUSHIMA 96012,JAPAN

来源：

FORENSIC SCIENCE INTERNATIONAL | 1995年 / 76卷 / 01期

关键词：

semen identification; seminal gamma-glutamyl transpeptidase; monoclonal antibodies; enzyme-linked immunosorbent assay;

D O I：

10.1016/0379-0738(95)01794-1

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

A sandwich enzyme-linked immunosorbent assay (ELISA) was developed for detecting human seminal gamma-glutamyl transpeptidase (gamma-GTP) using a combination of anti-seminal gamma-GTP monoclonal antibodies. These monoclonal antibodies did not react with human ovary or uterus in immunohistochemical study. Optimal assay condition, resulting in a sensitive assay with a low background, is presented. The detection limit of this assay was estimated to be 1 ng/ml of seminal gamma-GTP corresponding to about 100 000 times dilution of seminal sample. This ELISA was specific for seminal gamma-GTP, without cross-reactivity to renal or hepatic gamma-GTP, normal blood serum, non-coital vaginal fluid or saliva. The recovery of seminal gamma-GTP added to various biological fluids were also examined.

引用

页码：35 / 45

页数：11

共 20 条

[1] PURIFICATION AND IMMUNOLOGICAL CHARACTERIZATION OF A NEW FORM OF GAMMA-GLUTAMYL-TRANSFERASE OF HUMAN SEMEN [J].